N., and Bakry, N. M. (2006)

Language: English
English
Publication Type: Journal Article
Classification: X 24180 Social poisons & drug abuse
Classification: F 06791 Experimental
Subfile: Immunology Abstracts; Toxicology Abstracts

Johnen, B. G. (1978). Rhizosphere microorganisms and roots stained with europium chelate and fluorescent brightener. Soil Biology and Biochemistry 10: 495-502.

Chem Codes: Chemical of Concern: DZ Rejection Code: METHODS.

Roots, root hairs and associated microorganisms were stained with a mixture of europium-(III)thenoyltrifluoroacetonate (Eu(TTA)3) and the disodium salt of 4.41-bis(4-anilino-6-bis(2-hydroxy-ethyl)amino-S-triazin-2-ylamino)2.21-stilbene disulphonic acid and fluorescent brightener (FB). Eu(TTA)3 stained cells containing nucleic acid, resulting in red fluorescence, and FB stained dead cells and organic matter, which fluoresced green.Differentiation between root tissue and microbial cells was achieved by applying the FB stain directly to soil or growth solution during the last 2 days of growth before slide preparation. The FB stain was readily absorbed by the roots during this period. Subsequent staining with Eu(TTA)3 for 30 min was sufficient to thoroughly stain the microorganisms associated with the roots and root hairs. During this period, Eu(TTA)3 did not penetrate into the root tissue to any great extent. Microorganisms which fluoresced bright red could therefore be distinguished easily from root tissue, which fluoresced green. Variable degrees of red or green fluorescence, which were brought about by different age of root and subsequent differences in stain absorption, could he compensated for by adjusting the microscope filter combination to favour either FB or Eu(TTA)3 fluorescence.The method was used to obtain information on the effect of the insecticide diazinon and of several plant growth regulators on the rhizosphere microflora and to study the efficiency of methods to remove microorganisms from the root surface

Johnson, K. A. and Weisskopf, C. P. (1997). The Use Of Passive Sampling Devices For The Assessment Of Soil Pesticide Residues. 213: Agro 49.
Chem Codes: CHLOR Rejection Code: METHODS.

biosis copyright: biol abs. rrm meeting abstract pesticides pollution methodology soil science passive sampling methods site assessment alachlor herbicide atrazine metolachlor chlorpyrifos organophosphorus pesticide diazinon terbufos pcb congeners chlorinated organics polychlorinated biphenyl congeners dieldrin p p'-ddt methoxychlor field method general biology-symposia, transactions and proceedings of conferences, congresses, revie/ methods, materials and apparatus, general-field methods/ toxicology-environmental and industrial toxicology/ public health: environmental health-air, water and soil pollution/ soil science-general/ methods (1970- )/ pest control, general/ pesticides/ herbicides

Johnson, N. C. and Pfleger, F. L. (1992). Vesicular-Arbuscular Mycorrhizae and Cultural Stresses. In: G.J.Bethlenfalvay and R.G.Linderman (Eds.), ASA (Am.Soc.of Agron.), Spec.Publ.No.54, Oct.31, 1991, Denver, CO, Am.Soc.of Agron.Inc., Crop Sci.Soc.of Am.Inc., Soil Sci.Soc.of Am.Inc., Madison, WI 71-99.
Chem Codes: EcoReference No.: 70839
Chemical of Concern: SZ,PNB,CBL,DZ,PRN,CBF,ADC,DCNA,PHMD Rejection Code: REFS CHECKED/REVIEW.

Johnson, R., Tietge, J., Stokes, G., and Lothenbach, D. (1993). The Medaka Carcinogenesis Model. In: Tech.Rep.9306, Compendium of the FY1988 & FY1989 Res.Rev.for the Res.Methods Branch, U.S.Army Biomedical Res.& Dev.Lab., Ft.Detrick, Frederick, MD 147, 172 (U.S.NTIS AD-A272667).

EcoReference No.: 17126

Chemical of Concern: ADC,DXN,ASCN,FML,BNZ,ISO,AN,24DXY,PL,DDT,NAPH,ISO,DPDP,Se,TOL,MXC,DCB,DZ; Habitat: A; Effect Codes: MOR,CEL,GRO; Rejection Code: LITE EVAL CODED(ASCN),NO ENDPOINT(NAPH,DZ,ADC).

Johnson, W. E., Fendinger, N. J., and Plimmer, J. R. (1991). Solid-Phase Extraction Of Pesticides From Water: Possible Interferences From Dissolved Organic Material. 63: 1510-1513.

Chem Codes: Chemical of Concern: SZ, CHLOR Rejection Code: CHEM METHOD .

biosis copyright: biol abs. a multiresidue analysis for trifluralin, simazine, atrazine, propazine, diazinon, parathion-methyl, alachlor, malathion, parathion, chlorpyrifos, pendimethalin, methidathlion, and def in water that utilizes liquid-solid extraction (lse) with octadecyl-bonded silica cartridges (c18bscs) followed by gas chromatography/mass spectrometric analysis was developed. recoveries of most pesticides were greater than 80% with c18bscs from fortified water at concentration levels from about 1 to 500 ppb. reco humic acid solution (10 ppm dissolved organic carbon) made to simulate a natural water with a high dissolved organic content, ranged from 29 to 153% and in general were lower than recoveries obtained from pure water. 14c-labeled diazinon and parathion were recovered from the humic acid solution at levels of 57 and 68%, respectively, with c18bscs; the remainder of the labeled pesticides was found in the cartridge eluents. partition coefficients with humic acid were calculated based on recovery of 14c-labeled pesticides from the c18bscs. ecology/ environmental biology-general/ methods/ ecology/ environmental biology-oceanography and limnology/ biochemical methods-general/ biochemical studies-general/ toxicology-general/ methods and experimental/ toxicology-environmental and industrial toxicology/ public health: environmental health-air, water and soil pollution/ pest control, general/ pesticides/ herbicides

Johnson, W E, Fendinger, N J, and Plimmer, J R ( 1991). Solid-phase extraction of pesticides from water: possible interferences from dissolved organic material. Analytical Chemistry 63: 1510-1513.
Chem Codes: Chemical of Concern: SZ Rejection Code: CHEM METHOD.

A multiresidue analysis for trifluralin, simazine, atrazine, propazine, diazinon, parathion-methyl, alachlor, malathion, parathion, chlorpyrifos, pendimethalin, methidathion, and DEF in water that utilizes liquid-solid extraction (LSE) with octadecyl-bonded silica cartridges (C18BSCs) followed by gas chromatography/mass spectrometric analysis was developed. Recoveries of most pesticides were greater than 80% with C18BSCs from fortified water at concentration levels from about 1 to 500 ppb. Recoveries with C18BSCs, from an optically adjusted humic acid solution (10 ppm dissolved organic carbon) made to simulate a natural water with a high dissolved organic content, ranged from 29 to 153% and in general were lower than recoveries obtained from pure water. 14C-Labeled diazinon and parathion were recovered from the humic acid solution at levels of 57 and 68%, respectively, with C18BSCs; the remainder of the labeled pesticides was found in the cartridge eluents. Partition coefficients with human acid were calculated based on recovery of 14C-labeled pesticides from the C18BSCs. [Journal Article; In English; United States] http://www.sciencedirect.com/science/article/B6WVB-45CP509-4MS/2/c71f63d671ab501f1b6f61db9d52e4d8

Johnson, W. E., Fendinger, N. J., and Plimmer, J. R. (1990). SOLID PHASE EXTRACTION OF PESTICIDES FROM WATER POSSIBLE INTERFERENCES FROM DISSOLVED ORGANIC MATERIAL. 200th American Chemical Society National Meeting, Washington, D.c., Usa, August 26-31, 1990. Abstr Pap Am Chem Soc 200 : Agro 56.
Chem Codes: Chemical of Concern: SZ Rejection Code: CHEM METHOD.

ABSTRACT: BIOSIS COPYRIGHT: BIOL ABS. RRM ABSTRACT TRIFLURALIN SIMAZINE ATRAZINE PROPAZINE DIAZINON PARATHION-METHYL ALACHLOR MALATHION PARATHION CHLORPYRIFOS PENDIMETHALIN METHIDATHION INSECTICIDE GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHYLENE CHLORIDE LIQUID-LIQUID EXTRACTION

KEYWORDS: General Biology-Symposia
KEYWORDS: Radiation-Radiation and Isotope Techniques
KEYWORDS: Ecology
KEYWORDS: Biochemical Studies-General
KEYWORDS: Biophysics-General Biophysical Techniques
KEYWORDS: Pest Control
KEYWORDS: Economic Entomology-Chemical and Physical Control

Johnson, W. W. and Finley, M. T. (1980). Handbook of Acute Toxicity of Chemicals to Fish and Aquatic Invertebrates. Resour.Publ.137, Fish Wildl.Serv., U.S.D.I., Washington, D.C 98 p. (OECDG Data File) (Publ As 6797).

Chem Codes: EDT,RSM,Captan,CBF,CBL,DFZ,PSM,24DXY,ACP,ACR,AZ,BS,Captan,CMPH,CPY,DBN,DMB,DMT,DPDP,DS,DU,DZ,FO,GYP,HCCH,HXZ,MDT,MLN,MLT,MOM,MP,Naled,OYZ,PRT,SZ,TBC,TPR Rejection Code: PUBL AS.

Quell#915//Diazinon DRAFT/REFs 1999//Lead DRAFT/REFs 1999//Parathion '86//Duplicate data with 6797 deleted, ALP 08/03//

EcoReference No.: 666
User Define 2: ECOTOX NA,REPS,WASH,CALF,CORE
Chemical of Concern: EDT,RSM,Captan,CBF,CBL,DFZ,PSM,24DXY,ACP,ACR,AZ,BS,Captan,CMPH,CPY,DBN,DMB,DMT,DPDP,DS,DU,DZ,FO,GYP,HCCH,HXZ,MDT,MLN,MLT,MOM,MP,Naled,OYZ,PRT,SZ,TBC,TPR
Endpoint: MOR,ITX,BEH

Johnson, W. W. and Finley, M. T. (1980). Handbook of Acute Toxicity of Chemicals to Fish and Aquatic Invertebrates. Resour.Publ.137, Fish Wildl.Serv., U.S.D.I., Washington, D.C 98 p. (OECDG Data File) (Publ As 6797).

Chem Codes: EcoReference No.: 666
Chemical of Concern: EDT,RSM,Captan,CBF,CBL,DFZ,PSM,24DXY,ACP,ACR,AZ,BS,Captan,CMPH,CPY,DBN,DMB,DMT,DPDP,DS,DU,DZ,FO,GYP,HCCH,HXZ,MDT,MLN,MLT,MOM,MP,Naled,OYZ,PRT,SZ,TBC,TPR,As,Pb Rejection Code: PUBL AS.

Johnston, G. (1995). The Study of Interactive Effects of Pollutants: A Biomarker Approach. Sci.Total Environ. 171: 205-212.

EcoReference No.: 59499

Chemical of Concern: PCZ,MLN,CYP,DMT,DZ; Habitat: T; Effect Codes: BCM; Rejection Code: OK(MLN,CYP),NO CONTROL(DMT,DZ),MIXTURE(PCZ).

Johnston, G., Walker, C. H., and Dawson, A. (1994). Interactive Effects Between EBI Fungicides (Prochloraz, Propiconazole and Penconazole) and OP Insecticides (Dimethoate, Chlorpyrifos, Diazinon and Malathion) in the Hybrid Red-Legged Partridge. Environ.Toxicol.Chem. 13: 615-620.

EcoReference No.: 67235

Chemical of Concern: DMT,DZ,CPY,MLN,PCZ; Habitat: T; Effect Codes: BCM; Rejection Code: NO MIXTURE(DMT,PCZ),NO ENDPOINT(DZ).

Johnston, G., Walker, C. H., and Dawson, A. (1994). Interactive effects between EBI fungicides (prochloraz, propiconazole and penconazole) and OP insecticides (dimethoate, chlorpyrifos, diazinon and malathion) in the hybrid red-legged partridge. Environmental Toxicology and Chemistry [ENVIRON. TOXICOL. CHEM.]. Vol. 13, no. 4, pp. 615-620. 1994.

Chem Codes: Chemical of Concern: DZ Rejection Code: MIXTURE.

ISSN: 0730-7268

Descriptors: toxicity
Descriptors: fungicides
Descriptors: insecticides
Descriptors: organophosphorus compounds
Descriptors: Alectoris rufa
Abstract: The toxicokinetic interactions between the ergosterol-biosynthesis-inhibiting (EBI) fungicides prochloraz, propiconazole, and penconazole and the organophosphorus (OP) insecticides dimethoate, chlorpyrifos, and diazinon have been studied in the hybrid red-legged partridge. The inhibition of serum butyrylcholinesterase (BuChE) activity provided a useful biochemical indicator of the generation of the toxic oxon metabolites of these OP insecticides. Birds pretreated with 180 mg/kg prochloraz tended to show a greater inhibition of serum BuChE activity at 1, 4, and 24 h following oral exposure to either of the OPs dimethoate (3 mg/kg) or chlorpyrifos (9 mg/kg) compared to birds pretreated with corn oil. Prochloraz-pretreated birds also showed a tendency toward an increased inhibition at 24 h following dosing with the OP diazinon (4.3 mg/kg) compared to corn oil controls. In the case of dimethoate, the inhibition of serum BuChE activity was significantly greater in treated birds than controls at 24 h. Birds pretreated with the EBI fungicide propiconazole (200 mg/kg) showed a similar inhibition of serum BuChE activity to those pretreated with corn oil following administration of 167 mg/kg malathion. Pretreatment with the EBI fungicide penconazole (200 mg/kg) produced significantly greater depression of serum BuChE activity at 1, 4, and 24 h after dosing with malathion, when compared to corn oil controls. The tendency toward increased inhibition of serum BuChE activity by each of the OPs in prochloraz-pretreated birds was attributed to an increased activation of the compound to its active oxon form as a consequence of induction of microsomal monooxygenases by prochloraz.
Language: English
English
Publication Type: Journal Article
Classification: P 6000 TOXICOLOGY AND HEALTH
Classification: X 24135 Biochemistry
Subfile: Toxicology Abstracts; Pollution Abstracts

Jokanovic, M. and Maksimovic, M. (1995). A Comparison of Trimedoxime, Obidoxime, Pralidoxime and HI-6 in the Treatment of Oral Organophosphorus Insecticide Poisoning in the Rat. Arch.Toxicol. 70: 119-123.

EcoReference No.: 74883

Chemical of Concern: DMT,DDVP,FNT,PPHD,FNTH,TCF,PRIM,DZ,PRT,DEM,AZ,DPY,PSM,PHSL,MLN; Habitat: T; Effect Codes: MOR; Rejection Code: NO CONTROL(ALL CHEMS).

Jonas, R., Klockow, M., Lues, I., and Wurziger, H. (1994). Stereoselectivity of the positive inotropic effects of newer diazinone-cardiotonics. Bioorganic & Medicinal Chemistry Letters 4: 2585-2588.

Chem Codes: Chemical of Concern: DZ Rejection Code: METHODS.

In 5-methyl-dihydropyridazinones, a stereoselectivity is observed only with respect to the phosphodiesterase III-inhibition. The (-)-enantiomers are very strong inhibitors whereas their (+)-counterparts exhibit only a weak activity. In the case of the thiadiazinone EMD 53998 a pronounced stereoselectivity regarding Ca-sensitivity and phosphodiesterase inhibition is apparent. It is concluded that Levosimendan exerts its positive inotropism almost exclusively through its potent phosphodiesterase III-inhibitory effect.

Jones, D. P. ( Taint Hazards In Pesticides.
Chem Codes: Chemical of Concern: DCNA Rejection Code: NO TOX DATA.

hapab despite the wide assortment and large quantities of pesticides in use, the varying conditions of usage, and the numerous crops involved, instances of flavor impairment are rare, but do occur. the mixed isomers of bhc are known for the characteristic taint produced in several crops, notably potatoes, carrots and black currants. its persistance in soil can result in tainting many years after application. bhc should not be used on arabica coffee because of production of so-called bricky flavor. in amercian tests of 23 crops, bhc, lindane, toxaphene, endrin and malathion were individually associated with a depreciation of flavor. chlordane has been implicated in flavor loss in potatoes, and aldrin and dieldrin, in carrots; a fusty flavor in strawberries is attributed to demeton-s- methyl. acaricides have been reported very infrequently in flavor impairment, although genite 923 is likely to cause changes in peaches and chlorfenson in pears, while dmc, chlorobenzilate and possibly diazinon may be responsible for flavor changes developing during storage. among the fungicides, an outstanding problem is the production of off-flavors by certain sulphur compounds in canned produce; lime-sulphur and the thiocarbamates are suspect here: examples are captan in canned and fresh strawberries, ferbam in black currants, thiram in canned and quick frozen black currants and canned and fren strawberries, nabam in canned black currants, dicloran in canned carrots and strawberries, dinocap in hops, and pcnb in potatoes. herbicides are not associated with as much flavor impairment as insecticides. residues and their monitoring 67/06/00, 15 1967 ai: yes db: tox sf: hapab

JONES FW and WESTMORELAND DJ (1999). Removal of wool wax, nonylphenol ethoxylates and pesticide residues from wool scour effluent. WATER RESEARCH; 33 1775-1780.
Chem Codes: Chemical of Concern: DZ Rejection Code: EFFLUENT.

BIOSIS COPYRIGHT: BIOL ABS. The aqueous scouring of raw wool produces a highly polluting effluent that primarily contains emulsified wool wax, together with high levels of nonionic detergents and trace levels of various agricultural pesticides. The SIROLAN CF chemical flocculation process transferred over 95% of the wool wax and detergent and greater than 98% of the pesticide residues from the wastewater stream to a spadeable sludge that was used as a starting material to produce a high quality compost. During the composti Biochemistry/ Public Health

Jones, K. H., Sanderson, D. M., and Noakes, D. N. (1968). Acute Toxicity Data for Pesticides (1968). World Rev.Pest Control 7: 135-143.

EcoReference No.: 70074

Chemical of Concern: 24DXY,ABT,ACL,ADC,AMTL,AMTR,AND,ASM,ATN,ATZ,AZ,BFL,BMC,BMN,BS,BTY,Captan,CBL,CCA,CHD,CMPH,CPP,CPY,CQTC,CTHM,Cu,CuFRA,DBN,DCB,DCNA,DDD,DDT,DDVP,DEM,DINO,DLD,DMB,DMT,DOD,DPP1,DQTBr,DS,DU,DZ,DZM,EDT,EN,EP,EPTC,ES,ETN,FLAC,FMU,FNF,FNT,FNTH,Folpet,HCCH,HPT,LNR,Maneb,MCB,MCPA,MCPB,MCPP1MDT,MLH,MLN,MLT,MRX,MTM,MVP,MXC,Naled,NPM,PB,PCH,PCL,PCP,PEB,PHMD,PHSL,PMT,PPHD,PPN,PPX,PPZ,PQT,PRN,PRO,PRT,PYN,PYZ,RTN,SFT,SID,SZ,TCF,TFN,THM,TRB,TRL,TXP,VNT,Zineb; Habitat: T; Effect Codes: MOR; Rejection Code: NO PUBL AS(24DXY,ABT,ACL,AMTL,AMTR,ASM,ATN,AZ,BFL,BMC,BMN,BS,BTY,CCA,CMPH,CPP,CPY,CQTC,CTHM,DBN,DCB,DCNA,DDT,DINO,DOD,DPP1,DQTBr,DU,DZM,EP,EPTC,ES,FMU,FNF,FNT,Folpet,HCCH,HPT,LNR,MCB,MCPP1,MLT,MP,MRX,MTM,MXC,Naled,NPM,Pb,PCH,PCL,PEB,PHSL,PPN,PPZ,PQT,PRO,PYN,PYZ,RTN,RYA,SFT,SID,TFN,THM,TRL,VNT),NO CONTROL,DURATION(ALL CHEMS).

Jones, Simon and Selitsianos, Dimitrios (2005). Stereochemical consequences of the use of chiral N-phosphoryl oxazolidinones in the attempted kinetic resolution of bromomagnesium alkoxides. Tetrahedron: Asymmetry 16: 3128-3138.

Chem Codes: Chemical of Concern: DZ Rejection Code: METHODS.

A number of chiral N-phosphoryl oxazolidinones have been prepared and evaluated as asymmetric phosphoryl transfer agents with the magnesium alkoxide of 1-phenyl ethanol. The reaction proceeded with little stereoselection, which was shown to be a consequence of the reaction mechanism that occurs with inversion of configuration at phosphorus consistent with in-line attack opposite the leaving group.

Joyce, B. A., Wallender, W. W., Angermann, T., Wilson, B. W., Werner, I., Oliver, M. N., Zalom, F. G., and Henderson, J. D. (2004). Using infiltration enhancement and soil water management to reduce diazinon in runoff. Journal of the American Water Resources Association [J. Am. Water Resour. Assoc.]. Vol. 40, no. 4, pp. 1063-1070. Aug 2004.
Chem Codes: Chemical of Concern: DZ Rejection Code: FATE.

ISSN: 1093-474X

Descriptors: Runoff
Descriptors: Pesticides
Descriptors: Soil Treatment
Descriptors: Infiltration
Descriptors: Storm Runoff
Descriptors: Diazinon
Descriptors: Orchards
Descriptors: Water Quality
Descriptors: Water Management
Descriptors: Toxicity
Descriptors: Nonpoint Pollution Sources
Descriptors: Irrigation Effects
Descriptors: Surface Water
Descriptors: Rivers
Descriptors: Water Pollution Control
Descriptors: Soil Water
Descriptors: Field Tests
Descriptors: Irrigation Practices
Descriptors: Stormwater runoff
Descriptors: Irrigation
Descriptors: Nonpoint pollution
Descriptors: USA, California, Central Valley
Abstract: Pesticide runoff from dormant sprayed orchards is a major water quality problem in California's Central Valley. During the past several years, diazinon levels in the Sacramento and San Joaquin Rivers have exceeded water quality criteria for aquatic organisms. Orchard water management, via post-application irrigation, and infiltration enhancement, through the use of a vegetative ground cover, are management practices that are believed to reduce pesticide loading to surface waters. Field experiments were conducted in Davis, California, to measure the effectiveness of these management practices in reducing the toxicity of storm water runoff. Treatments using a vegetative ground cover significantly reduced peak concentrations and cumulative pesticide mass in runoff for first flush experiments compared with bare soil treatments. Post-application irrigation was found to be an effective means of reducing peak concentrations and cumulative mass in runoff from bare soil treatments, but showed no significant effect on vegetated treatments.
Language: English
English
Publication Type: Journal Article
Classification: SW 3070 Water quality control
Classification: AQ 00002 Water Quality
Classification: P 2000 FRESHWATER POLLUTION
Classification: EE 40 Water Pollution: Monitoring, Control & Remediation
Subfile: Environmental Engineering Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 2: Ocean Technology Policy & Non-Living Resources; Meteorological & Geoastrophysical Abstracts; Pollution Abstracts; Aqualine Abstracts; Water Resources Abstracts

Jubb, G. L. Jr. (1984). Patterns of Pesticide Use on 'Concord' Grapes in Erie County, Pennsylvania: 1970-1982. Melsheimer Entomol.Ser. 34: 1-11.

Chem Codes: User Define 2: REPS,WASH,CALF,CORE,SENT,NA
Chemical of Concern: SZ,PRN,CBL,DZ,Cu Rejection Code: NO TOX DATA.

Jubb, G. L. Jr. (1984). Patterns of Pesticide Use on 'Concord' Grapes in Erie County, Pennsylvania: 1970-1982. Melsheimer Entomol.Ser. 34: 1-11.

Chem Codes: Chemical of Concern: SZ,PRN,CBL,DZ,Cu Rejection Code: NO TOX DATA.

Julien, Michel, Millot, Claire, Tocanne, Jean-Francois, and Tournier, Jean-Francois ( 1997). 12-O-Tetradecanoylphorbol-13-Acetate Inhibits Aminophospholipid Translocase Activity and Modifies the Lateral Motions of Fluorescent Phospholipid Analogs in the Plasma Membrane of Bovine Aortic Endothelial Cells. Experimental Cell Research 234: 125-131.

Chem Codes: Chemical of Concern: DZ Rejection Code: IN VITRO.

The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) is a potent mitogenic factor which can replace the growth promoting activity of basic fibroblast growth factor (bFGF) on bovine aortic endothelial cells. However, TPA-treated cells lose their strict contact inhibition at confluence, which is a characteristic of cells grown in the presence of bFGF. We have examined whether these changes could be related to modifications of the transbilayer and lateral motions of fluorescent lipids, namely 1-acyl-2-[6-[N-(7-nitrobenz2-oxa-1,3-diazol-4-yl)amino]caproyl]-phosphatidyl-choline (C6-NBD-PC), -phosphatidylserine (C6-NBD-PS), and -phosphatidylethanolamine (C6-NBD-PE) inserted in the outer leaflet of the cell plasma membrane. In TPA-treated cells, the three fluorescent phospholipids remained located in the outer leaflet for at least 1 h at 20[deg]C after their insertion, indicating a blockade of the aminophospholipid translocase activity which is normally present in the plasma membrane of bFGF-treated cells [1, 2]. TPA also induced a large increase in the percentage of C6-NBD-PC and C6-NBD-PE probes which were free to diffuse laterally. The mobile fractionsMreached values of ~100% for the two lipids, while for bFGF-treated cells they were found around 85 and 75%, respectively. For the C6-NBD-PS probe,Mremained unchanged in bFGF and TPA-treated cells, at around 85%. TPA treatment also induced a twofold increase in the lateral diffusion coefficients of C6-NBD-PC and C6-NBD-PE, while that of C6-NBD-PS remained nearly unchanged. These effects of TPA may be related to the observed loss of differentiated properties of vascular endothelial cells and not to its mitogenic properties.

Kabir, S. M. H. and Ahmed, N. (1979). Histopathological Changes in Climbing Perch, Anabas testudineus (Bloch) (Anabantidae: Perciformes) due to Three Granular Insecticides. Bangladesh J.Zool. 7: 21-29.

EcoReference No.: 6885