Bachelor degree

Module designation	Module 6. Molecular biology and genetic engineering Molecular biologу Genetic engineering
Module level, if applicable	Basic professional modules
Code, if applicable	MB 2417 GI 3418
Subtitle, if applicable	-
Courses, if applicable	Course		Credits
	Molecular biologу		3
	Genetic engineering		3
			2 lectures + 1 lab / week
			2 lectures + 1 seminar / week
Semester(s) in which the module is taught	4, 6 semester
Person responsible for the module	Dr., Professor Bisenbayev A.K., Dr.Professor Aytasheva Z.G.
Lecturer	Dr., Professor Bisenbayev A.K., Dr.Professor Aytasheva Z.G.
Language	Kazakh, Russian, English
Relation to curriculum	(State/Social/ Vocational/IET) Vocational
Type of teaching, contact Hours	Lecture	Practicum		Seminars
	30 hours per semester 2 hours per week	15 hours per semester 1 hour per week		-
	30 hours per semester 2 hours per week	-		15 hours per semester 1 hour per week
Workload	Lectures	Guided self-study		Self-study
	30 hours per semester 2 hours per week	-		60 hours per semester 4 hours per week
	30 hours per semester 2 hours per week	20 hours per semester		60 hours per semester 4 hours per week
Credit points	3 credits= 5 ECTS 3 credits= 5 ECTS
Requirements according to the examination regulations	It is necessary to have knowledge of basic terms and techniques in molecular biology and genetiс engineering.
Recommended prerequisites	Biochemistry, cytology and histology, genetics.
Module objectives/intended learning outcomes	Knowledge	Skills		Competences
	Remember: physical and chemical properties and levels of organization of pro- and eukaryotic genetic material; the basic research approaches in molecular biology; Understand: mechanisms of gene expression regulation; approaches and methods of recombinant DNA production and cloning; in vitro mutagenesis; selective down regulation of gene expression with antisense RNA, RNA interference.	Apply: skills of utilizing lab equipment; preparing lab reports; Analysis: different approaches and methods used for investigating a molecular process from molecular perspective.		Evaluate: all equipment and materials required for lab work; Create: recombinant DNA; common genetic vectors, methods of pro- and eukaryotic cell transformation; in vitro mutagenesis and clone selection.
Content	The course will cover the following topics:
	Molecular biologу: 1. Structure and forms of DNA and RNA; 2. Methods of its isolation; 3. Genome organization; 4, 5. DNA replication; 6, 7. Structure of the gene. Transcription; 8, 9. Features of genetic code; 10, 11. Translation; 12. Regulation oа gene expression on the level of transcription and translation; 13, 14. Mutations. Spontaneous and induced mutagenesis; 15. Mechanisms of DNA reparation.
	Genetic engineering: 1. History of the development of genetic engineering. General principles and methods of genetic engineering 2, 3. Methods for isolating and identifying genes. Methods of preparation for cloning genes 4. Vector system in prokaryotes. Expression vectors and cloning vectors 5, 6. Functional features of the structure of the genetic apparatus of prokaryotes. Comparative analysis of the organization of the genetic information and pro-and eukaryotic. 7. Creation of industrial producing strains by genetic engineering. Directed mutagenesis of the DNA molecules in vitro 8. Genetically engineered yeast system. Cloning of genes into cells of S. cerevisiae. 9. Expression vector system S. cerevisiae 10. Secretion of foreign proteins from cells S. cerevisiae. Production of foreign proteins in S. cerevisiae 11. Insect viruses as vectors of expression of foreign genes 12. Genetic transformation of mammalian cells. Introduction of DNA into mammalian cells 13. Transgenic animals in basic research. Biotechnological application of transgenic animals 14. Vector systems based on animal viruses. 15. Transgenic plants with new biotechnological properties. Transgenic plants in agriculture.
Study and examination requirements and forms of examination	1^st interim control: attendance, in-class discussion, problem solving, testing 2^nd interim control: attendance, in-class discussion, problem solving, testing Final examination: written, 2 theoretical questions, 1 practical question
Media employed	Video lectures, presentations
Reading list	References: Glik B., Pasternak J. Molecular biotechnology. Principles and apply. M., 2002 (in russian)

Module designation	Module 7. Industrial and Food Biotechnology Basics of biotechnological Industry
Module level, if applicable	Basic Professional Modules
Code, if applicable	BBI 3316
Subtitle, if applicable	-
Courses, if applicable	Course		Credits
	Basics of biotechnological Industry		3
			2 lectures + 1 seminar / week
Semester(s) in which the module is taught	5 semester
Person responsible for the module	Dr., Professor Zayadan B.K. PhD Akymbekov N.Sh.
Lecturer	Dr., Professor Zayadan B.K. PhD Akymbekov N.Sh.
Language	Russian, Kazakh, English
Relation to curriculum	Vocational
Type of teaching, contact Hours	Lecture	Practicum		Seminars
	30 hours per semester 2 hours per week	-		15 hours per semester 1 hour per week
Workload	Lectures	Guided self-study		Self-study
	30 hours per semester 2 hours per week	20 hours per semester		60 hours per semester (4 hours per week)
	Seminars
	15 hours per semester 1 hour per week
Credit points	3 US credit = 5 ECTS
Requirements according to the examination regulations	50% of overall scores
Recommended prerequisites	BMB 2414 Basics of Microorganisms Biotechnology
Module objectives/intended learning outcomes	Knowledge	Skills		Competences
	The students should know: technological scheme of production of microbial bio-products; principles of aseptic processing; techniques that provide an exception entry of microorganisms in the production process; the necessary hardware; gygiene requirements for production facilities and manufacturing processes. Remember Remember the broad knowledge in the fields of microbiology and virology, environmental biotechnology and etc. Understand Has the knowledge of the most recent researches, discoveries and application of microorganisms in industrial and food microbiology.	Must own the: methods of sterilization of media and equipment; skills with the cultures of microorganisms, maintenance and storage of production strains; ways of growing seed; methods of laboratory simulation of production processes. Apply Apply the rules of planning and optimization of production processes in industrial and food biotechnology Analysis Analyze, independently plan and perform experiments with industrial microorganisms		Be able to: get a yeast biomass; determine the energy of the yeast fermentation; get apple cider vinegar; get citric acid superficial and deep way; carry out solid-phase cultivation producing amylase. Evaluate Evaluate the obtained results and draw conclusions and use of the world scientific databases Create Create and interpret research results of physiological and biochemical analyses based on the obtained data
Content	The purpose of the course - learning the foundations of food microbiology and biotechnology industries. Sections of the course: the objects and processes of the biotechnological production; the security of biological objects and biotech industries; microbiological and technological bases of fermentation industries; technological scheme of production of the products of microbial synthesis; production, using viable microbial cells; immobilized biological objects; hardware, sanitary and hygienic requirements industrial equipment and manufacturing processes. Introduction and history of biotech; Profile of the biotechnology industry; Clustering in the biotechnology industry; White and green biotechnology; Industrial production systems based on microorganisms and cell cultures; Bioethanol, organic acids, antibiotics, industrial enzymes, recombinant proteins, monoclonal antibodies; Vaccine production; Enzyme technology
Study and examination requirements and forms of examination	Oral exam, tests 1^st interim control: attendance, in-class discussion, problem solving, testing 2^nd interim control: attendance, in-class discussion, problem solving, testing Final examination: written, 2 theoretical questions, 1 practical question
Media employed	Video lectures, Audio books, presentations
Reading list	Gladys Alexandre and etc. Advances in applied microbiology [2009]. ISBN: 978-0-12-374788-4 (in english) John Wiley & Sons Ltd. Dictionary of Microbiology and Molecular Biology, Third Edition [2006]. ISBN-13 978-0-470-03545-0 (in english) Talaro−Talaro: Foundations in Microbiology, Fourth Edition [2011]. ISBN - 978-0072320428 (in russian) Reinhard Renneberg. Biotechnology for Beginners [2007]. ISBN-9780123735812. Eugene W. Nester and etc. Microbiology: a human perspective, sixth edition [2011]. ISBN 978–0–07–299543–5 (in english) Prescott, Harley, and Klein’s microbiology, seventh edition [2008]. ISBN 978–0–07–299291–5