BACTERIOLOGICAL INDEXES OF INTESTINAL MICROFLORA
CHANGES IN HIV PATIENTS
Nuruzova Z.A.
Tashkent Medical Academy
In recent years the trends of fast spreading of HIV infections have been observed. Annually from, 4, 3 to 6, 6 million
people influence with HIV infection and more than 2 million people die from this disease. One of the main clinics of silent period
of HIV infection is a syndrome of prolonged diarrhea, which it can lead to dehydration and loss of weight of organism. In HIV
infection the connection of gastro – intestinal system to pathologic process occurs different terms of the disease. It can be
developed not only morphological disorders of the walls intestine but the decreasing of its resistance, development of intestinal
dysbacteriosis, severe course and development of infectious process that is capable to recurrence. The purpose of research.
To study the condition of dysbacteriosis of intestinal microflora in HIV patients. Scientific novelty. It has been studied for the
first time the large intestine microbiocenosis of HIV patients in Uzbekistan. The tasks of research. To reveal the degrees of
colon microbiocenosis condition and intestinal dysbacteriosis in patients with HIV. Material and methods. For investigation the
material were taken the results of patients, who have been admitted to the clinic of specific infectious diseases under the
Republican center of fighting against the AIDS and bacteriologic investigations were performed in bacteriologic laboratory of
this clinic. 17 patients with diarrhea syndrome II-III-IV sub clinic degree were selected for investigation and the degrees of
disease were marked on the basis of order № 81, March 4, 2015 of the Health Ministry of the Republic of Uzbekistan. From
them 8(47%) – men, 9(53%) – women, the age of them from 18-30(average 25). Patients faeces were diluted with physiologic
solution in 1:10 and Endo, blood agar, blood agar for bacteriods, MRE-4 (selective environment for reduction of milk) for lacto-
bacteria, Blaurocco for bifidobacteria, Saburo for fungi, Kitta-Tarotstsi for clostridia, Vilson-Bler conditions were planted by Gold
method and put into thermostat to 37° C, 18-24, 48-72 hours. Identification of microorganisms was carried out by general
standard methods. The results of research. After the bacteriologic investigation of II-degree patients’ faeces of dysbacteriosis
was revealed in patients. E. coli 10
5
CMU/g, quantity of lactose negative E. coli >10
6
, Lactobacillus spp.> 10
4
CMU/g, Bifidobac-
terium spp. >10
3
CMU/g, Bacteroid spp. >10
4
CMU
/g, Enterococcus spp. decreased than in norm, condition – pathogen mi-
crobes: Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus spp. 10
6
CMU / g, Candida >10
7
CMU
/g, and also the
quantity of pathogen hemolytic E. coli, Streptococcus spp., S. aureus agents were determined. Conclusion. 1. II-degree of
dysbacteriousis of intestinal microflora was observed in all stages of disease in patients with HIV infection. 2. The decrease of
general quantity of indigene microflora in patients, especially the quantity of bifido-, lacto bacteria, and bacterioids and condi-
tional-pathogen microorganisms decreased more than 2-3 times.
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