[Environment] Chief Editor Iftikhar A. Raja comsats institute of Information Technology Abbottabad (Pakistan)

Muhammad Asif^*, Zafar Iqbal^1*, Jan Alam^*, Shujaul M Khan^**, Farhana Ijaz^* & Inayat U Rahman^*

^* Department of Botany Hazara University Mansehra, Pakistan

^**Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan

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ABSTRACT

Medicinal ethno botany is the sub-discipline of ethno botany which refers to the study of conformist uses of plants and informal knowledge concerning to plants and human health care, as well as prevention and helpful of human ailment using plants. Ethno botanical evidence on salutary plants and their practices by intrinsic philosophies is supportive not only in the conservancy of traditional nations and biodiversity, but also for communal health care and remedy improvement. Traditional knowledge regarding the uses of local wild medicinal plants for treating various human diseases is totally in the hold community old members. The young generation is not much aware about such important traditional medicinal practices. An ethno medicinal study was to document and accumulate massive and disseminate indigenous knowledge of century’s practiced therapeutic uses of medicinal plants for medicinal purpose by the tribal people living in this area. Total 167 local inhabitants (123 men, 38 women and 5 herbalists) were interviewed through questionnaire. The data obtained were quantitatively analyzed through the use value (UV). Plant specimen was preserved and mounted on herbarium sheets and labeled, cataloging and deposited with voucher numbers in Hazara University Herbarium, Mansehra, Pakistan. 59 plant species belonging to 55 genera and 24 families. These medicinal plant species are used commonly as an ethno medicine. The herbaceous plant species were dominant with 70%, followed by shrubs (20%) and trees (10%). Lamiaceae was the leading family among collected medicinal plant species (16.94%). Maximum medicinal plant species were used for treatment of acidity (11.76%). Most widely part used is leaf (58%) for the treatment of different ailments by the traditional healers. The medicinal plant species with greater use value were Berberis parkerana Schneid. (0.87) ranked 1^st, Bergenia ciliata (Haw.) (0.81) 2^nd, Berberis pachycantha Royle (0.79) 3^rd. The current study supported to launch list of plants used in ethno medicine practices in study area. Future antimicrobial, antivirals, and pharmacological studies are required to ratify the efficacy and safety of the medicinal plants species.

DAE-58

IN VITRO EMRYOID DEVELOPMENT FROM ANTHERS OF DIFFERENT MAIZE GENOTYPES
Muhammad Zeshan^1,*, Zubair Ahmad*, Salma Perveen**and Saad Imran Malik

*PMAS-Arid Agriculture University, Rawalpindi

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ABSTRACT

In maize, hybrid development has been an efficient tool to improve yield since long time but the conventional way of inbred line development for hybrids is very slow process. However, the development of doubled haploid (inbred) line in vitro by pollen and anther culture can shorten this time span. As the development of haploids and doubled haploids is highly genotype dependent so for that, a preliminary study was carried out on six advance genotypes of maize to access their response to anther culture for embryoid development. Two nutritional medium; N6 and YP were used for culturing anthers of these six genotypes. Significant difference of genotypes was found for responding anthers as well as for embryoid frequency. Genotype 77-80 gives the best response for anther response with a percentage of 35.24 on YP media and 19.98 on N6 media. This genotype also significantly differs from all the others for number of embryoids per anther. For embryoid frequency N6 media gives better response with 1.466 embryoids per anther but on YP media it is 1.302. Genotype 85-88 was found recalcitrant with no response on both media. These results indicate that anther response to tissue culture is highly genotype dependent so this is an important factor for the development of plantlets through anther culture. This experiment provides the basis for selection of suitable genotype and media for further studies.

DAE-59

SCREENING OF SORGHUM (SORGHUM BICOLOR L.) GENOTYPES UNDER VARIOUS LEVELS OF DROUGHT STRESS.
Masood Qadir^*, Amir Bibi and Hafeez Ahmad Sadaqat

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad.

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ABSTRACT

Sorghum is an important fodder crop and plays an important role in the Pakistan dairy industry. High yielding and superior quality sorghum varieties should be produced to meet the domestic needs. In arid and semiarid regions, drought is a major and serious constraint to sorghum production and adversely influences sorghum growth and germination. There are many approaches to combat the drought stress. Among these approaches breeding of crops contributes towards increase in yield under stress condition like drought stress by making the plant tolerant against stress. Ten genotypes of sorghum were evaluated at seedling stage to determine the genotypic variation among them on the basis of tolerance against drought stress and impact of drought on fodder quality. Three levels of drought stress (100%, 50% and 75% field capacity) were applied to the genotypes. The experiment was carried out in wire house following a triplicate completely randomized design with factorial arrangements. The data was recorded after 20 days of sowing on following traits such as root and shoot length, root shoot fresh and dry weight, leaf area, relative water content, total ash contents and crude protein. All the traits showed significant difference among the genotypes. The genotypes NARC-11 followed by Sorgh-11 gave better response in all levels of drought stress while F114 gave poor response in all levels of drought stress.

DAE-70

GROWTH AND METAL UPTAKE POTENTIAL OF GREEN GRAM INFLUENCED BY NICKEL RESISTANT BACTERIA AT VARYING NICKEL STRESSES

Syeda S. Meryem*, A. Yasmin

* Department of Environmental Sciences COMSATS institute of Information Technology, Abbottabad,

Fatima Jinnah Women university, Rawalpindi, Department of Environmental Sciences

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