Chapter- 4 : Reagents (solution) used in clinical laboratories



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chapter 4 reagents

Dilution…..

  • Diluting body fluids and calculating dilution.
  • In the laboratory it is frequently necessary to dilute body fluids.
  • Diluting body fluids
  • Examples: To make 8ml of a 1 in 20 dilution of blood:
  • Volume of blood required 8 = 0.4ml
  • 20
  • There fore to prepare 8 ml of a 1 in 20 dilution add 0.4ml of blood to 7.6ml of diluting fluid.
  • Exercise
  • If it desired to make 500ml of 0.2M solution of HCl from a 1.00 molar stock solution. How can we make it?
  •  

4.4. Reagent solutions

    • A reagent or reactant is a substance or compound consumed during a chemical reaction.
    • A reagent is more specifically "a test substance that is added to a system in order to bring about a reaction or to see whether a reaction occurs".
    • Such a reaction is used to confirm the presence of another substance.

Reagent …

    • Reagent
      • Any substance employed to produce a chemical reaction. In medical laboratory, this is used in conjunction with a given specimen to produce a measurable or noticeable change.
      • The accuracy of tests depends to a great extent on the accuracy of the reagents used.

4.4.1. Types of reagent solutions

  • 1. Stock reagent solution
    • Is a concentrated reagent solution which is diluted to prepare a working solution.
    • Has a longer shelf life and occupies less space in storage than the working solution.
  • 2. Working –reagent solution
    • Can be diluted from the stock solution or prepared directly from the reagent chemical following the recommended procedures.

Types of reagent …

  • 3. Saturated solution
    • Is a solution in which further solute can not be dissolved
    • A saturated solution must have the deposits of the solute at the bottom of the reagent bottle.

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