Chapter- 4 : Reagents (solution) used in clinical laboratories


Quantitative expressions…



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chapter 4 reagents

Quantitative expressions…..

  • a. Percentage weight by weight % w/w
        • This is the number of parts of solute by weight per 100 parts solution by weight.
    • Example: - 37gm%w/w HCL means each 100 gm of HCL solution contain 37gm of HCL and the rest 63 gm solvent (water)

Quantitative expressions…..

  • b. Percentage weight by volume (%w/v)
  • It is the number of parts of solvents by weight per 100 parts of solution by volume.
  • c. Percentage volume by volume % V/V
    • It is the number of parts of solute by volume per 100 parts of solute.
  • Example :- 70% V/V ethanol means,70ml of ethanol was mixed with 30ml of water.

Exercises -1

    • How do you prepare an aqueous solution of 30gm% W/V NaOH?
    • Prepare 4 liter of 20% W/V trichloroacetic acid (TCA).

Quantitative expressions…..

  • 2. Parts per unit.
      • This is to express the number of parts by volume or by weight of solute per given volume or weight of the solution.
  • Exercise-2
  • If you are asked to prepare 1000ml of a mixture of acetic acid and methanol. If the procedure says mix 1 parts of acetic acid with 3part of methanol. How can you prepare?

Quantitative expressions…..

  • B. Chemical units.
  • Commonly used chemical unit to express the quantity of solutes in a given solution are:-
      • Mole
      • Equivalents
      • Molarity
      • Normality
      • Molality
  • 1. Mole.
    • 1 mole of a give substance is its atomic weight or molecular weight expressed in gram.

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