Chapter- 4 : Reagents (solution) used in clinical laboratories

Chapter- 4 : Reagents (solution) used in clinical laboratories

Acknowledgment

• Addis Ababa University
• Jimma University
• Hawasa University
• Haromaya University
• University of Gonder
• American Society for clinical pathology
• Center for disease control

Objective.

• After completion of this chapter, the student will be able to:
• Define different terminologies related to reagent & chemicals.
• know different methods of expression of strength of solution
• Carry out different calculations.
• Describe safe handling of chemicals & reagents.
• Explain about standard & staining solutions .

Out line

• 4.1. Definitions
• 4.2. Types of solution
• 4.3 Expression of the strength of solution
• 4.4. Reagent solutions
• 4.5. Grades of chemicals
• 4.6. Standard solutions
• 4.7. Staining solutions

4 : Reagents (solution) used in clinical laboratories

• 4.1. Definitions:
• Solution
• A solution: - is a homogenous mixture of two or more components that can be varied in composition within certain limit.
• Every solution consists of two parts, the solvent and solute
• Solvent: - is the substance that dissolves the solute. The solvent in most cases are liquid.

Reagents (solution) ……………..

• Liquid solvent are divided in two:-
• Polar solvents, like water, alcohol
• Non-polar solvent like chloroform,benzene,ether,CCl4

Reagents (solution) ……………..

• Solute:-is the substance that is dissolved in solvent, the solute can be solids, liquid or gases.
• If a solid or gas is mixed with a liquid, the liquid is considered as solvent.
• If the solution is a mixture of two liquid, the component present in large amount is considered as the solvent.