This Catalog is a compilation of equipment at the six Israel nano centers available to all users on a fee basis: internal users, researchers at other universities and industry

Instrument	Description	Equipment Location	Operator'>Technion Faculty		Academia	Industry	Operator	Faculty in Charge/Link	Contact email	Contact phone
			Operator	Self
NanoWizard II, BioAFM	AFM has crossed over from its roots as a technology for nanoscopic measurement tasks in material sciences to establish major AFM application fields in the life sciences: High Resolution Imaging – Scanning Electron Microscopy resolution on untreated samples; Force Measurements down to the single molecule; Stiffness and Elasticity Measurements – the mechanical response of a surface over a large range of forces provides stiffness or adhesion information level – single bond forces are easily accessible; Nanomanipulation/Lithography – the AFM tip used as a nanoscopic manipulator.	The Russell Berrie Nanoparticles and Nanometric Systems Characterization Center	בימים אלה מתבצע תהליך של הערכת עלויות ואלה יפורסמו בעתיד. Sabina Prilutsky (Ifraimov) 04-8292116					http://www.technion.ac.il/~eshimoni/	http://nnscc.technion.ac.il/	04-8292484
Philips CM120 and an FEI Tecnai T12 G2	Transmission electron microscopes. dedicated to cryogenic work. The two microscopes are equipped with CCDs cameras: wide-angle Gatan Multiscan791 cameras on both, and a high-resolution US1000 camera on the FEI microscope. Three cryo-holders and their transfer-stations are available.	Electron Microscopy of Soft Matter	290 ₪	160 ₪	ללא מפעיל - 160 ₪ עם מפעיל - 290 ₪	ללא מפעיל 300 ₪ כולל מפעיל 500 ₪	Judith Schmidt	http://www.technion.ac.il/~ceritit/	[e-mail]	04-8292845
Rheometric Scientific ARES rheometer	Equipped with a 100FRTN1 transducer and cone-and-plate, parallel-plates and Couette measuring devices.	Electron Microscopy of Soft Matter	220 ₪	80 ₪	ללא מפעיל - 80 ₪ עם מפעיל - 220 ₪	ללא מפעיל - 230 ₪ עם מפעיל - 340 ₪	Judith Schmidt	http://www.technion.ac.il/~ceritit/	[e-mail]	04-8292845
Zeiss Ultra Gemini HR-SEM	High resolution scanning electron microscope equipped with Espirit EDS x-ray microanalysis system and cryo-SEM	Electron Microscopy of Soft Matter	290 ₪	160 ₪	ללא מפעיל - 160 ₪ עם מפעיל - 290 ₪	ללא מפעיל 300 ₪ כולל מפעיל 500 ₪	Judith Schmidt	http://www.technion.ac.il/~ceritit/	[e-mail]	04-8292845
Leica UC6 Ultramicro-tome FC6	Equipped with an FC6 cryo-chamber for cryo-ultramicrotomy	Electron Microscopy of Soft Matter					Judith Schmidt	http://www.technion.ac.il/~ceritit/	[e-mail]	04-8292845
Olympus BH-2	Light microscope system equipped with Nomarski, phase-contrast and cross-polarizers optics, and with an Optronics CCD camera.	Electron Microscopy of Soft Matter	80 ₪	90 ₪	ללא מפעיל - 80 ₪ עם מפעיל - 90 ₪	ללא מפעיל - 180 ₪ עם מפעיל - 270 ₪	Judith Schmidt	http://www.technion.ac.il/~ceritit/	[e-mail]	04-8292845

Instrument	Description	Equipment Location	Technion Faculty		Academia	Industry	Operator	Faculty in Charge/Link	Contact email	Contact phone
			Operator	Self
Small-angle x-ray scattering (SAXS)	Small-angle x-ray scattering (SAXS) is a well-established technique for studying structural features of colloidal or nanometric dimensions (1-100 nm). Appropriate analysis of SAXS data can provide wealth of structural information, on nano-scale objects, synthetic or biological, such as size, shape, internal structure and mass of particles, degree of agglomeration or superstructure, particle size distribution in polydispersed systems, and fractal dimensions in disordered systems. Some of the research areas which can be approached by this technique: Polymer solutions and gels. Biopolymer and bioactive materials in solution. Colloids and surfactant systems. Nanoparticles in liquid dispersion, powder or composite. Ceramics, sol-gel glasses. Zeolites. Semicrystalline polymers. Polymer blends. Block copolymers. Polymer fibers (including nanofibers). Composite (and nanocomposite) materials. The small-angle X-ray scattering facility at the Technion operates the following equipment:	Chemical Eng. Faculty					Rafail Khalfin	Yachin Cohen	[e-mail]	8292113
	Kratky system: Philips PW1730 sealed tube X-ray generator (with Cu anode), Kratky Compact collimation (A. Paar) with temperature controllers and a linear position sensitive detector (Raytech) coupled to a Nucleus multichannel analyzer. This set-up is considered a “one dimensional” system which is appropriate for isotropic samples, without preferred orientation (or for systems with very high orientation for which it gives information on the cross-section perpendicular to the orientation direction).		$2.5/hr or $10/hr including data analysis	$60/day or $10/hr including data analysis	$60/day or $10/hr including data analysis	$10/hr including data analysis
	Bruker system: a sealed tube generator (Kristalloflex K760-80), cross coupled Göbel Mirrors and A.Paar HR-PHK high-resolution X-ray small-angle pinhole chamber. The detector is a two-dimensional Bruker Nanostar multi-wire area detector, with associated electronics and processors. With this system anisotropic structures can be evaluated, such as oriented block copolymers or colloidal crystals. It is also possible to measure x-ray diffraction at wide angles, be decreasing the sample-detector distance from about 65 cm to about 5 cm. Sample holders for liquid and solid samples , with temperature control in the ranges of -20 to +70 oC and ambient to 300 oC (A. Paar KPR and KHR, respectively) are in use in both instruments.		$60/day or $10/hr including data analysis
	J.J system: A new combined Small/Wide angle scattering system designed by Molecular Metrology. It includes an Osmic MicroMax 002 source with confocal max-flux optics 3 pinhole collimation, 2-D multiwire detector (Gabriel design, 200mm dimension), Linkam TMHS600 temperature controller for -170 to 300 °C. For wide-angle x-ray diffraction Fuju image plate and reader are used, and can be measured simultaneously with SAXS measurements		$60/day or $10/hr including data analysis

Instrument	Description	Equipment Location	Technion Faculty		Academia	Industry		Faculty in Charge	Contact email	Contact phone
			Operator	Self
Bl-200SM Research Goniometer System (Brookhaven Instruments)	Goniometer System (Brookhaven Instruments, Inc.), it designed for both static and dynamic light scattering measurements. The instrument is equipped with a Compass 415M solid state laser generating light of 532 nm wavelength (green). It enable to measure molecular weight, particle size distribution, radius of gyration, second virial coefficient, etc.	The Russell Berrie Nanoparticles and Nanometric Systems Characterization Center					Sabina Prilutsky (Ifraimov)	http://nnscc.technion.ac.il/	[e-mail]	04-8292116
FluoroLog 3-22- Modular Spectrofluorometer (Horiba Scientific, Ltd.)	FluoroLog 3-22 Spectrofluorometer that provide the fluorescence measurements with very high sensitivity, allowing detecting very low concentration of fluorescence. Moreover it scans the data in very fast way, avoiding sample decomposition due to the photobleaching, or other reasons depended on time. Our FluoroLog 3-22 Spectrofluorometer is also equipped by Polarizers for SpexR spectrofluorometers and the F-3000 Fiberoptic Adaptor modules. Using the Polarizer for SpexR spectrofluorometers fluorescence polarization and anisotropy can be measured. This provides an information about the rotational behavior of molecules that relates to their molecular size and shape, and to viscosity of the medium. The aim of F-3000 Fiberoptic Adaptor is to perform the measurements for samples that can not be placed inside the sample compartment. It also has Auto Titration Injector F-3006 and MicroMax 384 Microwell-Plate Reader that able to automate and facilitate the measurements.	The Russell Berrie Nanoparticles and Nanometric Systems Characterization Center	122 ₪	61 ₪	ללא מפעיל - 61 ₪ עם מפעיל - 122 ₪	ללא מפעיל - 84 ₪ עם מפעיל - 168 ₪	Sabina Prilutsky (Ifraimov) 04-8292116	http://nnscc.technion.ac.il/	[e-mail]	04-8292116
NanoWizard® II BioAFM (JPK Instruments AG)	The principle of AFM imaging can be resumed as follows: a sharp silicon probe is moved on a surface by means of a controlled piezo-based system. The probe interacts with the sample surface, and the probe deflections are recorded through the deflections of a laser beam, which reflects from the probe top side and is collected on photo-detector. The signals from the detector and the feed-back piezo-based system are used for the image reconstitution by electronic means. The topography of the sample is recorded with the nanometric resolution. Other sample characteristics, origin from the probe-surface interactions, are acquired simultaneously with the topography in the different imaging modes and demonstrated as images. NanoWizard® II BioAFM enable to measure the topography of the sample with a nanometric resolution. It is also equipped with the different accessories that provide him same additional features. It is possible to make a measurements in controlled liquid environment (flow, temperature), which can be critical for living cell experiments. Kelvin probe microscopy technique can be also used to image samples presenting a distribution of electrical properties on inhomogeneous materials; to probe phase separation, chemical recognition and molecular orientation.	The Russell Berrie Nanoparticles and Nanometric Systems Characterization Center					Sabina Prilutsky (Ifraimov) 04-8292116	http://nnscc.technion.ac.il/	[e-mail]	04-8292116

Instrument	Description	Equipment Location	Technion Faculty		Academia	Industry	Operator	Faculty in Charge/Link	Contact email	Contact phone
			Operator	Self
BI-DNDC: Differential Refractometer	The BI-DNDC is a deflection type refractometer that may be purchased for use in either batch or GPC/SEC mode. In batch mode, the specific refractive index increment, dn/dc, is determined. This value is required as a parameter in molecular weight measurements using light scattering. In GPC/SEC mode, dn/dc is already known, and the instrument is used as a concentration detector for GPC/SEC applications.	The Russell Berrie Nanoparticles and Nanometric Systems Characterization Center					Sabina Prilutsky (Ifraimov) 04-8292116	http://nnscc.technion.ac.il/	[e-mail]	04-8292116
Multiphoton Multispectral Laser-Scanning Microscope (Zeiss LSM 510 META NLO)	The Laser Scanning Confocal Imaging System is a microscope that uses lasers to visualize fluorescent markers. With this system one obtains images by scanning a laser beam of light over a specimen. Multiphoton laser-scanning microscopy (MPLSM) and multispectral laser-scanning microscopy (MSLSM) provide a fundamental advantage over standard confocal laser–scanning microscopy by enhancing the ability to collect images in multiple dimensions. Using near infrared radiation (NIR) excitation combined with non-linear optics (NLO) in which two or more photons excite the fluorophore almost-simultaneously. MPLSM further allows data collection deeper into the sample and more images can be obtained over longer times.	Technion Life Sciences and Engineering Infrastructure Unit located in the Faculty of Biology	25$	-	25$	50$	-	http://isu.technion.ac.il	[e-mail]	04-8295834
	Uses of MPLSM and MSLSM. 1) Analyses of deeper portions of tissues and organs expressing multiple fluorophores within developing organisms. 2) Live imaging with reduction in phototoxic effects allows longer exposures to analyse cellular processes in vivo and in vitro. 3) Because of the NLO it is possible to excite and image fluorochromes that are normally excited only in the UV range (e.g. Hoechst, DAPI). Multiphoton and multispectral laser-scanning microscopy allows high-resolution multicolor multidimensional imaging of cells inside light-scattering tissues eliminating many restrictions of the confocal microscope.
4-Dimensional Microscope for Living Cells (Zeiss Cell Observer Imaging System)	This optical microscopic system is ideal for imaging of tissue culture cells using transmitted light, Nomarski optics and epi-fluorescence. The system includes an inverted state-of-the-art microscope, motorized specimen stage, incubation and cell cultivation with control of CO2, temperature and humidity, two high resolution cooled CCD cameras and AxioVision software to record time lapse, Z-stacks, multichannel acquisition, multi dimentional aquisition in XYZ, deconvolution and other modules. “Real time” imaging of live samples is possible since it is not necessary to fix and section the specimens providing the ability to obtain high quality optical imaging of thick sections in three dimensions and over time. The images are recorded directly into the hard drive of a computer that also controls the microscope.	Technion Life Sciences and Engineering Infrastructure Unit located in the Faculty of Biology	10$	-	10$	20$	-	http://isu.technion.ac.il	[e-mail]	04-8295834