Ishni bajarish tartibi. Asosiy standart eritma tayyorlash uchun kimyoviy toza NH4Fe(SO4)2*12H,O temir achchiqtoshidan tarozida 0,5190 g tortib olib, u hajmi 100 ml bo'lgan kolbaga solinadi. Eritmani nordonlashtirish uchun kolbaga 10 ml suyultirilgan (1:1) sulfat kislota va belgisigacha distillangan suv quyiladi. Bunda standart eritmaning konsentratsiyasi 0,06 mg/ml bo'ladi. So‘ngra sulfosalitsil kislota va ammiakning 10 % li eritmalari tayyorlanadi. Keyin beshta standart ishchi eritma quyidagicha tayyorlanadi: beshta 100 ml hajmli kolbaga asosiy standart eritmadan 1,2, 3,4 va 5 ml o'lchab quyiladi. Har bir kolbaga sulfosalitsil kislota va ammiak eritmalaridan ham 10 ml dan qo‘shiladi. Kolbalarga belgisigacha distillangan suv quyilib, eritmalar yaxshilab aralashtiriladi. Standart eritmalarning erituvchiga nisbatan optik zichliklari λ=416 nm to'lqin uzunligida besh martadan o'lchanadi. Ishlatiladigan erituvchini tayyorlashda 100 ml hajmli kolbaga 10 ml sulfosalitsil kislota, 10 ml ammiak eritmalari quyiladi va kolba belgisigacha distillangan suv bilan to'ldiriladi. O'lchash natijalarining o‘rtachasi topilib, eritmaning optik zichligi va konsentratsiyasi orasidagi bog'lanishni ifodalovchi darajalash chizmasi tuziladi.
Tarkibida 0,1 dan 0,6 mg gacha temir bo'lgan namuna 100 ml sig'imli kolbaga o'tkaziladi. Unga 10 % sulfosalitsil kislota va ammiak eritmalaridan 10 ml dan quyiladi, hamda distillangan suv qo‘shib eritma sathi kolbaning belgisiga qadar yetkaziladi. Shundan so‘ng tekshiriladigan eritmaning optik zichligi o'lchanadi va uning konsentratsiyasi topiladi. Olingan natijalaryordamida namunadagi temirning umumiy miqdori hisoblanadi.
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№8 laboratoriya ishi
