SylbV01-00-00. pmd

VII. Practical
• Seed morphology and structure;

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142
Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
• Desiccation rates and freezing to low and ultra low temperatures, seed storage
behavior determination in sample seeds, seed viability and vigour tests;
• Seed longevity and accelerated ageing test in different types of seeds, handling
hard seededness and physiological immaturity;
• Post harvest handling methods of difficult-to-store seeds, dormant buds, and pollen,
ultra-desiccation of seeds, biochemical tests of seed deterioration;
• Preparation of stock solutions, culture media, cryoprotectant solutions and regrowth
media, Isolation of explants and 
in vitro
culturing in growth retarding media for
slow growth conservation, meristem isolation in dicots and monocots;
• Pretreatments, preculturing, cryoprotectant treatments varying temperature and
durations, cold hardening- plants and explants, cryopreservation techniques-
encapsulation-dehydration, vitrification, encapsulation-vitrificaion, droplet
freezing, thawing- slow and fast, recovery and regrowth- media, light conditions;
•
In vitro-
cryo-genebanking and database management, morphological and molecular
markers for assessing genetic stability-demonstration.
VIII. Teaching methods
• Lectures
• Power point presentations
• assignments, quiz
• Group tasks, student’s presentations
IX. Learning Outcome
Advanced conservation techniques including biotechnological tools would be learnt
by students.
X. Suggested Reading
Barbara MR, Chin HF and Normah MN. 2013. Conservation of Tropical Plant Species. Springer.
Bewley JD and Black M. 1994. 
Seeds Physiology of Development and Germination
, Second
Edition. Plenum Press, New York and London.
Chaudhury R and Malik SK. 2017. 
Cryopreservation of Plant Species: Practical Approaches
from Handling to Cryobanking
. ICAR-NBPGR, New Delhi. 52 p.
Chaudhury R, Pandey R, Malik SK, Bhag Mal (eds). 2003. 
In vitro
Conservation and
Cryopreservation of Tropical Fruit Species. IPGRI Office for South Asia, New Delhi, India/
NBPGR, New Delhi, India, 293 pp.
Cromarty A. 1984. Techniques of drying seeds, pp 88-125. 
Seed Management Techniques for
Genebank 
(JB Dicke, S Linington and JT Williams, eds). International Board on Plant
Genetic Resources, Rome.
Cromarty A, Ellis RH and Robert EH. 1982. 
The Design of Seed Storage Facilities for Genetic
Conservation
, Revised 1985. International Board on Plant Genetic Resources, Rome.
Ellis RH, Hong TD and Roberts EH. 1985a. Handbook of Seed Technology for Genebank Volume
II. Principles and Methodology. International Board for Plant Genetic Resources, Rome.
Ellis RH, Hong TD and Roberts EH. 1985b. Handbook of Seed Technology for Genebank
Compendium of Specific Germination Information and Test Recommendations.
International Board for Plant Genetic Resources, Rome.
Ellis RH. 1988. The viability equation, seed viability monographs, and practical advice on seed
storage. 
Seed Science and Technology
16
: 29-50.
Hong TD and Ellis RH. 1996. A protocol to determine seed storage behaviour. International
Plant Genetic Resources Institute IPGRI Technical Bulletin No. 1, Rome.
Mandal BB, Chaudhury R, Engelmann F, Bhag Mal, Tao KL and Dhillon BS (editors). 2003.
Conservation Biotechnology of Plant Germplasm. NBPGR, New Delhi, India/ IPGRI, Rome,
Italy, 293 pp.

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Plant Sciences–Plant Genetic Resources
143
Reed BM. 2008. Cryopreservation—Practical Considerations. In: Reed B.M. (eds) Plant
Cryopreservation: A Practical Guide. Springer, New York, NY
Roberts EH. 1972. 
Viability of Seeds
. Chapman and Hall, London.