SylbV01-00-00. pmd

Total Credit Hours
100
Comprehensive (Pre-qualifying) Examination
(Non-credit of 100 marks) Satisfactory/ Not satisfactory
*Compulsory major courses

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Plant Sciences–Plant Genetic Resources
141
Course Contents
Ph.D. in Plant Genetic Resources (PGR)
I. Course Title
: Recent Advances in Germplasm Conservation*
II. Course Code
: PGR 601
III. Credit Hours
: 2(1+1)
IV. Why this course ?
Students need to understand all the recent and current issues and procedures for
germplasm conservation
V. Aim of the course
To provide knowledge on advances in seed physiology, biology and banking to lead
to retention of high seed quality during conservation and all aspects of conservation
science and technology.
VI. Theory
Unit I
Seed development and maturation; Seed storage behavior: physiological and
molecular basis of desiccation sensitivity; Dormancy, seed germination- mobilization
of reserves and their control processes; Viability and vigour-principle and testing
procedures; Seed testing for inadvertent introduction of transgenes.
Unit II
Seed storage for long-term conservation and factors affecting seed longevity; seed
processing for short, medium and long-term storage, artificial aging and controlled
deterioration test; ultra-desiccation techniques for germplasm conservation
, 
richness
index, ecological correlates of 
ex-situ 
seed longevity, permafrost conservation,
maintenance of Seed Genebank, status of global seedgene banks.
Unit III
In-vitro
techniques in PGR management, 
In-vitro
methods of clonal propagation,
In-vitro
collecting and germplasm exchange, Meristem culture and virus elimination,
somaclonal variation, application of somatic embryogenesis in PGR, Methods of 
in-
vitro
conservation- short, medium-term and long term, Concept of active and base
in-vitro 
genebank, Status of World cryo- and cryo-gene banks, embryo rescue
technique, history and principles of cryopreservation, cryoprotectants- role and
applicability, freezing injury and factors affecting cryoprotection, methods of
cryopreservation-conventional and vitrification based techniques, varied applications
of cryopreservation, handling difficult-to store non-orthodox seeds, embryonic axes,
pollen and dormant buds, Management of 
in-vitro
, cryo and DNA genebank- Practical
considerations, Monitoring genetic stability of 
in-vitro
conserved and cryopreserved
germplasm, database management for 
in-vitro
and cryopreserved germplasm