Сборник содержит материалы научной мультиконференции «Биотех- нология медицине будущего»

DNA aptamer incorporated surface sandwich bioassays for potential 
biomedical applications
Lee H.J.
Department of Chemistry, Kyungpook National University, Daegu 41566, South Korea
Surface sandwich bioaffinity sensing platforms have become an indispensable bioana-
lytical tool for the rapid screening and detection of biological and chemical molecules in real 
fluidic samples. In this presentation, I will discuss our recent efforts made on the development 
of highly selective bioaffinity chip platforms in conjunction with DNA aptamer and various 
shapes and sizes of gold and carbon nanoparticles for biological applications. Three different 
detection metholodogies including surface plasmon resonance, voltammetry and colorimetry 
were utilized. The first part of my talk will include the development of different sets of antibody-
aptamer or aptamer-aptamer bioreceptor pair sandwich assays specific for target analytes 
including disease diagnosis biomarkers such as protein tyrosine kinase 7 [1], alpha-1-anti-
trypsin [2], tau 381 [3], amyloid-beta(1-42) [4], as well as noro [5] and avian influenza [6] 
virus proteins in biological fluids in addition to some of commonly recognized diseases related 
metabolites. The second part of my talk will highlight our latest development on lateral flow 
based bioaffinity sensing platforms in conjunction with biofunctionalized nanoparticles and 
surface sandwich complex formation for protein biomarker molecules. Finally, future aspects 
and challenging issues of our aptamer based bioaffinity sensing platforms will be suggested.
1. Lee S., Hayati S., Kim S., Lee H.J. Determination of protein tyrosine kinase-7 concentration 
using electrocatalytic reaction and an aptamer-antibody sandwich assay platform. // Catal. 
Today. – 2019. – in press, doi: 10.1016/j.cattod.2019.05.029
2. Zhu G., Lee H.J. Electrochemical sandwich-type biosensors for α-1 antitrypsin with carbon 
nanotubes and alkaline phosphatase labeled antibody-silver nanoparticles. // Biosens. 
Bioelectron. – 2017. – V. 89. – p. 959–963.
3. Kim S., Wark A.W., Lee H.J. Femtomolar Detection of Tau proteins in Undiluted plasma 
Using Surface plasmon Resonance. // Anal. Chem. – 2016. – V. 88. – p. 7793–7799.
4. Diba F.S., Kim S., Lee H.J. Electrochemical immunoassay for amyloid-beta 1–42 peptide 
in biological fluids interfacing with a gold nanoparticle modified carbon surface. // Catal. 
Today. – 2017. – V. 295. – p. 41–47.
5. Kim S., Lee S., Lee H.J. An aptamer-aptamer sandwich assay with nanorod-enhanced 
surface plasmon resonance for attomolar concentration of norovirus capsid protein. // Sens. 
Actuators B. – 2018. – V. 273. – p. 1029–1036.
6. Diba F.S., Kim S., Lee H.J. Amperometric bioaffinity sensing platform for avian influenza 
virus proteins with aptamer modified gold nanoparticles on carbon chips. // Biosens. 
Bioelectron. – 2015. – V. 72. – p. 355–361.

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Всероссийская мультиконференция с международным участием «Биотехнология – медицине будущего»
29 июня - 2 июля 2019 г., г. Новосибирск, Россия
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