Сборник содержит материалы научной мультиконференции «Биотех- нология медицине будущего»

Fluorescent nucleic acid systems: design, construction, and application
Byeang Hyean Kim
Department of Chemistry, Division of Advanced Materials Science, pohang University
of Science and Technology (pOSTECH), 77 Cheongam-Ro, Namgu, pohang, Gyeongbuk, 
37673, Korea
Fluorescent nucleic acid systems are widely applied in various fields, from fundamental 
biological probes to nano-construction [1]. Currently fluorescent oligonucleotides play an 
important role in analysis of the genetic information and biosensing such as single nucleotide 
polymorphism (SNP) typing. Fluorescent nucleic acid systems encompass an extensive and 
exciting research area in chemistry as well as in biotechnology and photophysics. We synthesized 
and investigated new fluorescent nucleic acid systems for probing SNPs [2], structural changes 
of DNA [3], G- quadruplexes [4], and i-motifs [5], and applied to various photophysical devices.
We also investigated the fluorescent phenomena and structural aspects of pyrene modified
oligodeoxyadenylates. The covalently linked pyrenes induced the formation of a self- assembled 
oligodeoxyadenylate duplex, namely, A-cluster with unique fluorescent behavior [6].
1. Venkatesan N., Seo Y. J., Kim B. H. Quencher free Molecular Beacons: A New Strategy in 
Fluorescence based Nucleic Acids Analysis. // Chemical Society Reviews. - 2008. - V. 37. -
p 648-663.
2. Hwang G. T., Seo Y. J., Kim B. H. A Highly Discriminating Quencher-Free Molecular Bea-
con for probing DNA. // J. Am. Chem. Soc. - 2004. - V. 126. - p 6528-6529.
3. Kim K. T., Kim H. W., Moon D. H., Rhee Y. M., Kim B. H. DNS C: A Fluorescent, Environ-
mentally Sensitive Cytidine Derivative for the Direct Detection of GGG Triad Sequences. // 
Org. Biomol. Chem. - 2013. - V. 11. - p 5605-5614.
4. (a) Takahashi S., Kim K. T., podbevsek p., plavec J., Kim B. H., Sugimoto N. Recovery of 
the Formation and Function of Oxidized G-Quadruplexes by a pyrene-Modified Guanine 
Tract. // J. Am. Chem. Soc. - 2018. –V. 140. - p 5774-5783. 
5. park J. W., Seo Y. J., Kim B. H. Fluorescence modification of the AAAA (4A) loop: Toward 
a probe of the structural dynamics of the i-motif of the retinoblastoma gene. // Chem. Com-
mun. - 2014. - V. 50. - p 52-54.
6. (a) Seo Y. J., Rhee H., Joo T., Kim B. H. Self-Duplex Formation of an Apy-substituted 
Oligodeoxyadenylate and Its Unique Fluorescence. // J. Am. Chem. Soc. -2007. -V. 129. - p 
5244-5247. (b) Ro J. J., Lee H. J., Kim B. H. pyA-Cluster system for detection and imaging 
of miRNAs in living cells through double-three-way junction formation. // Chem. Com-
mun. - 2018. - V. 54. - p 7471-7474.
This work was supported by the National Research Foundation (NRF) of Korea (2015M3A9B8029067, 
2017R1A2A1A18071086). I am grateful to the colleagues named in the cited papers from my labo-
ratory and would like to thank professors N. Sugimoto, J. plavec and their coworkers for the fruitful 
collaborations.

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Всероссийская мультиконференция с международным участием «Биотехнология – медицине будущего»
29 июня - 2 июля 2019 г., г. Новосибирск, Россия
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