viii) Bacterial growth Bacterial growth rate in bioweathering experiments was monitored by frequent measurements
of optical density (OD
600
) using UV spectrophotometer (CADAS 50S, Dr Lange) at a
wavelength of 600 nm. The pH measurements were done using a (GLP 22) pH meter.
CHAPTER 3: MATERIALS AND METHODS
88
ix) Visual MinteQ modeling The geochemical simulation for bio/weathering experiments was performed using Visual
MinteQ modeling software (version 3.1 http://vminteq.lwr.kth.se/) in order to determine the
degree of saturation of the leaching solutions with respect to possible secondary precipitates.
The modeling was performed using the chemical composition of the leaching solution for
every incubation time.