Bachelor degree

1. Daniel Scherman. Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology: Principles, Delivery and Pharmacological and Biomedical Applications of Nucleotide-Based Therapies (Icp Textbooks in Biomolecular Sciences). - Imperial College Press, 2013, 400 p.

2. Bertram Katzung, Susan Masters, Anthony Trevor. Basic and Clinical Pharmacology. - McGraw-Hill Medical, 2011, 1248 p.

3. Robert A. Meyers. Pharmacology. - Wiley-Blackwell, 2008, 1126 p.

James M. Parry, Elizabeth M. Parry. Genetic Toxicology: Principles and Methods (Methods in Molecular Biology). – Humana Press, 2011, 452 p.

Module designation	Modules of Individual educational direction /traectory (IED) Stem cells biotechnology
Module level, if applicable	Module of Individual Educational Trajectory 4: "Genetics technology"
Code, if applicable	SCB 4510
Subtitle, if applicable	not applicable
Courses, if applicable	Course		Credits
	Stem cells biotechnology		3
			2 lectures + 1 seminar / week
Semester(s) in which the module is taught	7 semester
Person responsible for the module	PhD, Assoc. Prof. Dzhansugurova L.B.
Lecturer	PhD, Assoc. Prof. Dzhansugurova L.B., PhD, Assoc. Prof. Zhumabayeva B.A.
Language	Kazakh, Russian, English
Relation to curriculum	(State/Social/ Vocational/IET) IET
Type of teaching, contact Hours	Lecture	Practicum		Seminars
	30 hours per semester (2 hours per week)	-		15 hours per semester (1 hour per week)
Workload	Lectures	Guided self-study		Self-study
	30 hours per semester (2 hours per week)	20 hours per semester		60 hours per semester (4 hours per week)
	Seminars
	15 hours per semester (1 hour per week)
Credit points	3 credits= 5 ECTS
Requirements according to the examination regulations	Knowledge of differentiation mechanisms, marking of stem cells, prospects of application of cellular technologies in various areas of medicine, including an organ transplantation, test of medicines.
Recommended prerequisites	BAB 2416 Basics of Animal Biotechnology
Module objectives/intended learning outcomes	Knowledge	Skills		Competences
	Definition of the concept "stem cell" and its main properties, general characteristic and properties of embryonic stem cells, sources of stem cells for the therapeutic purposes, basic elements of eukaryiotic cellular cycle machinery, etc. Remember: potentially effective use of cellular biotechnology for scientific and practical purposes; Understand: creation of genetically modified stem cells and their use for study of mechanisms of implementation of genetic information during processes of morphogenesis and cellular differentiation.	Methods of study of transcription activity of separate components of the cellular cycle machinery, reporter proteins. Apply: modern methods of animal biotechnology; Analysis: mechanisms of differentiation.		Ability to design experiments and conduct research on stem cells biotechnology with the latest techniques. Evaluate: prospects of application of cellular technologies in various areas of medicine; Create: awareness of the ways for obtaining regenerates and their transformation.
Content	The course will contain the following topics:
	Stem cells biotechnology: 1. Concept of a stem cell. Definition of the concept "stem cell" and its main properties. Hypothesis of the mechanism of regulation of self-maintenance of stem cells. 2. Embryonic stem cells. General characteristic and properties of embryonic stem cells. Markers of embryonic stem cells. 3. Approaches to bioengineering designing of cellular lines of embryonic stem cells with the set properties for the medicinal purposes. 4. Ethical issues in study of stem cells. Sources of stem cells for the therapeutic purposes. 5. Ethical dilemma when using human embryonic stem cells. Induced pluripotent stem cells. 6. Basic elements of cellular cycle machinery and their role in regulation of cell division. Phases of a cellular cycle. 7. Role of separate elements of cellular cycle machinery. Basic elements of eukaryiotic cellular cycle machinery. 8. Intracellular and extracellular control of cellular division and growth. 9. Role of family of a product of a gene of a retinoblastoma in control of a cellular cycle, differentiation and apoptosis in somatic and stem cells. 10. Structure and functions of chromatin. Features of chromatin organization in embryonic stem cells. 11. Structural organization of control area of eukaryotic gene. Mechanisms of interaction between proteins of pRb u E2F families. 12. Role of regulation of transcription in operation of the cellular cycle machinery. 13. Methods of study of transcription activity of separate components of the cellular cycle machinery. Methods of reporter proteins. 14. Signal pathways of Bmi1 u Shh in regulation of functions of stem cells. 15. Somatic and tumor stem cells and their classification. Stem cells of skin and application in medicine. Concluding remarks.
Study and examination requirements and forms of examination	1^st interim control: attendance, in-class discussion, problem solving, testing 2^nd interim control: attendance, in-class discussion, problem solving, testing Final examination: written, 2 theoretical questions, 1 practical question
Media employed	Flash and ppt presentations
Reading list	References (all in English): 1. Joseph Panno. Stem Cell Research: Medical Applications and Ethical Controversies. - Checkmark Books, 2010, 262 p. 2. Robert Lanza et al. Essentials of Stem Cell Biology. Academic Press, 2009, 680 p.