Xxiv международная медико-биологическая конференция молодых исследователей Фундаментальная наука и клиническая

ACTIONS OF POLYPHENOLIC COMPOUNDS ON 
GLUTAMATE-BINDING SITES OF THE NMDA RECEPTOR 
SYNAPTOSOME IN THE RAT BRAIN
Khoshimov N.N., Rakhimova G.L., Mukhtorov A.A., Rakhimov R.N.
Institute of Biophysics and Biochemistry 
at the National University of Uzbekistan,
Namangan State University,
Institute of Bioorganic Chemistry named after acad. A. Sadykov 
Academy of Sciences of Uzbekistan,
Tashkent, Uzbekistan
Academic supervisor: Nasirov K.E., D. sci. (Biology), Prof.
Calcium ions have unique properties and a universal ability to conduct 
a variety of signals that have a primary effect on the cell. In each of the 
body’s signal transduction systems, Ca
2+
ions are important as a secondary 
mediator. Calcium is an effective regulator of metabolic processes in 
all cells, where there are systems that respond to small changes in its 
concentration. Calcium has long been known as an important link in 
synaptic transmission. Depolarization of the presynaptic nerve ending 
during the action potential opens voltage-gated calcium channels through 
which calcium enters the cell and triggers the release of the mediator from 
the vesicles. 
The effect of polyphenolic compound 7 (PC-7) on the neurotransmitter 
system of NMDA receptors in the membranes of rat brain synaptosomes 
was studied.
The synaptosomes were purified from the rat brain by two-stage 
centrifugation. Measure the amount of intracellular calcium [Ca
2+
]in was 
calculated by the Grinkevich equation.
In the presence of chlortetracycline (CTC) in the incubation medium, 
glutamate at a concentration of 50 μM increases the level of fluorescence, 
which indicates an increase in the concentration of Ca
2+
in the cytosol 
[Ca
2+
]in, caused by glutamate, due primarily to [Ca
2+
]in. Pre- the 
fluorescence level, then the addition of glutamate (50 μM) reduced the 
fluorescence and, accordingly, the level of [Ca
2+
]in in synaptosomes. The 
activity of the modulating effect of PC-7 on glutamate with synaptosomes 

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ФАРМАКОЛОГИЯ, ФАРМАКОГНОЗИЯ, ФАРМАЦЕВТИКА, ТОКСИКОЛОГИЯ
in rat brains was assessed in two ways: based on the bound glutamate on 
the concentration of PC-7 and study of the kinetics of glutamate binding 
in the presence of PC-7. Studies have shown that PC-7 effectively inhibits 
glutamate. The practical significance of the research results lies in the fact 
that the methods used and the identified mechanisms will serve as the 
basis for the determination of new neuroprotective compounds in order 
to create drugs and can be used as activators and inhibitors of the calcium 
channels of the nerve cell. 

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ФАРМАКОЛОГИЯ, ФАРМАКОГНОЗИЯ, ФАРМАЦЕВТИКА, ТОКСИКОЛОГИЯ
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