SylbV01-00-00. pmd

I. Course Title
: Biotechnology of Silkworm
II. Course Code
: SER 513
III. Credit Hours
: 1+1
IV. Why this course ?
Silkworm breeding by conventional methods takes relatively longer time. Combining
different desirable traits in to one individual requires breaking linkage between
desirable and undesirable traits. Application of biotechnology is essential to achieve
early results in silkworm crop improvement.

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Sericulture
767
V. Aim of the course
The course is aimed to provide knowledge on biotechnological methods and their
application in silkworm crop improvement. The course will address the available
methods and approaches that can be applied in the field of sericulture.
The course is organized as follows:
No
Blocks
Units
1
Biotechnological tools
1.
Tissue culture techniques
2.
Biotechnological methods
2
Application of biotechnology
1.
Molecular characterization and mapping
in silkworm
2.
Transgenics, Bioinformatics and biosafety
VI. Theory
BLOCK 1: Biotechnological tools
Unit 1:
Tissue culture techniques
Development ofpolyploids, gametoclonal variations - their scope and
applications. Cryopreservation.
Unit 2:
Biotechnological methods
Biotechnology and its scope in silkworm, recombinant DNA technology,
genes transfer systems-vector mediated gene transfer, microinjection,
electroporation, direct DNA uptake, gene gun technique, selectable
markers and reporter system. Molecular markers.
BLOCK 2: Application of biotechnology in silkworm
Unit 1:
Molecular characterization and mapping
Mulberry silkworm germplasm characterization by using molecular
markers, Development of maps, QTL mapping, MAS for economically
important traits in silkworm, Mapping populations (F2S and back
crosses RILs, NILs, DHs), Molecular mapping and tagging of
economically important traits.