SylbV01-00-00. pmd

I. Course Title
: Biotechnology of Mulberry
II. Course code
: SER 505
III. Credit Hours
: 1+1
IV. Why this course ?
Mulberry is perennial and highly heterozygous crop. Hence, selection in segregating

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Sericulture
759
progenies is very difficult. In order to aid the selection in mulberry, the
biotechnological tools, viz., Tissue culture techniques, molecular markers and
recombinant DNA technology are more useful to aid in selection. Application of
these techniques will shorten the breeding procedure. Hence this customised course.
V. Aim of the course
The course is designed to provide both basic and applied knowledge on the subjects
of Tissue culture techniques, molecular markers and recombinant DNA technologies
to PG students.
The course is organised as follows:
No.
Blocks
Units
1
Mulberry biotechnology, scope and
I
Mulberry biotechnology, scope and prospects
prospects
II
Mulberry germplasm characterization
III Genotyping and phenotyping
2
Recombinant DNA technology
I
Genes transfer systems
II
QTL mapping
III Seri bioinformatics
VI. Theory
BLOCK 1: Mulberry biotechnology, scope and prospects
Unit 1:
Mulberry biotechnology, scope and prospects
Scope of breeding for hardier and productive mulberry genotypes.
Preservation of genetic material. Development of transgenic mulberry.
Molecular linkage map of mulberry. Micropropagation:
 In-vitro
propagation in mulberry-production of haploids- induction of haploids,
advantages and disadvantages of haploids. Double haploids-induction,
advantages and disadvantages of double haploids. Practical
achievements of DH method, polyploids- Somaclonal variations,
Procedures, advantages, disadvantages and their applications.
Secondary metabolites. Gametoclonal variations – their scope and
applications. Cryopreservation: Definition and meaning, Steps in
cryopreservation, Advantages and disadvantages, Cryopreservation
Requirements, applications of cryopreservation in mulberry for
germplasm preservation.