SylbV01-00-00. pmd

VIII. Teaching methods
• Lectures,
• Power point presentations,
• assignments, quiz,
• Group tasks, student’s presentations
IX. Learning Outcome
Knowledge and skill gain on current basic and advanced methodologies in genetics
X. Suggested Reading
Griffin HG and Griffin AM. 1994. 
PCR Technology: Current Innovations
. CRC Press, London.
Hancock JF. 2004. 
Plant Evolution and Origin of crop species
, 2nd edition. CABI.
Henry RJ (Editor). 2001. 
Plant Genotyping: The DNA Fingerprinting of Plants
. Publisher: CABI
Publishing.
Karp A, Isaac PG and Ingram DS. 1998. 
Molecular Tools for Screening Biodiversity – Plants
and Animals
. Chapman and Hall, London.
Miller AJ. 2007. 
Crop Plants: Evolution
. John Wiley and Sons.
I. Course Title
: Principles of Plant Breeding for PGR Management
II. Course Code
: PGR 511
III. Credit Hours
: 2(1+1)
IV. Why this course ?
Students need to understand all the basic principles of plant breeding to be able
to exploit the PGR.
V. Aim of the course
To impart theoretical knowledge and practical skills about plant breeding objectives
in PGR management especially for germplasm maintenance, regeneration and pre-
breeding.
VI. Theory
Unit I
Objectives of plant breeding, genetic basis of breeding self- and cross – pollinated
crops, nature of variability, components of variation, genotype-environment
interaction, general and specific combining ability, self-incompatibility and male
sterility in crop plants and their commercial exploitation.
Unit II
Principles of breeding for biotic and abiotic stresses, Breeding self pollinated and
cross pollinated crops, pure line theory; pure line selection and mass selection
methods, line breeding, pedigree, bulk, backcross, single seed descent and multiline
method. Breeding methods in asexually/ clonally propagated crops, clonal selection.

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Plant Sciences–Plant Genetic Resources
137
Concept of plant ideotype and its role in crop improvement. Participatory Plant
Breeding, Plant breeders’ rights and regulations for plant variety protection and
farmers rights, DUS testing.
Unit III
Molecular breeding-molecular markers, fundamental concepts in the development
of molecular markers, types (isozymes, RFLP, RAPD AFLP), mapping populations
(RILs, NILs, DH, Backross), their merits and demerits, markers assisted selection,
linkage disequilibrium and the concept of marker-trait association-case studies,
marker assisted pre-breeding programmes.
VII. Practical
• Floral biology in self and cross pollinated species, selfing and crossing techniques;
• Selection methods in segregating populations and evaluation of breeding material.
• Analysis of variance (ANOVA);
• Estimation of heritability and genetic advance, maintenance of experimental
records;
• Learning techniques in hybrid seed production using male-sterility in field crops.