Restriction enzyme

BLNI-RO Bln I (Avr II)

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Bu sahifa navigatsiya:

• DPNI-RO Dpn I
• MAEIII-RO Mae III
• NCOI-RO Nco I
• NHEI-RO Nhe I
• NOTI-RO Not I
• PVUI-RO Pvu I
• B8781 Restriction Enzyme Buffer SB
• SACI-RO Sac I (Sst I)
• SFII-RO Sfi I
• SMAI-RO Sma I
• SPEI-RO Spe I
• 11417959001 SuRE/Cut ™ Buffer A
• 11417991001 SuRE/Cut ™ Buffer H
• 11417983001 SuRE/Cut ™ Buffer M
• XBAI-RO Xba I
• References 1. Arber W, Linn S. 1969. DNA Modification and Restriction. Annu. Rev. Biochem.. 38(1):467-500. http://dx.doi.org/10.1146/annurev.bi.38.070169.002343
• Restriktsiya mexanizmlarini o’rganishning ahamiyati

BLNI-RO	Bln I (Avr II)from Brevibacterium linens
Expand
DPNI-RO	Dpn Ifrom Diplococcus pneumoniae			Expand
MAEIII-RO	Mae IIIfrom Methanococcus aeolicus PL-15/H			Expand
NCOI-RO	Nco Ifrom Rhodococcusspecies (formerly Nocardia corallina)			Expand
NHEI-RO	Nhe Ifrom Neisseria mucosa			Expand
NOTI-RO	Not Ifrom Nocardia otitidis-caviarum			Expand
PVUI-RO	Pvu Ifrom Proteus vulgaris			Expand
B8781	Restriction Enzyme Buffer SB10 ×			Expand
SACI-RO	Sac I (Sst I)from Streptomyces achromogenes			Expand
SFII-RO	Sfi Ifrom Streptomyces fimbriatus			Expand
SMAI-RO	Sma Ifrom Serratia marcescens Sb			Expand
SPEI-RO	Spe Ifrom Sphaerotilus species			Expand
11417959001	SuRE/Cut™ Buffer ApH 8.1-8.3 (37 °C), suitable for molecular cloning			Expand
11417991001	SuRE/Cut™ Buffer Hsolution, pkg of 5 × 1 mL			Expand
11417983001	SuRE/Cut™ Buffer M			Expand
XBAI-RO	Xba Ifrom Xanthomonas campestris			Expand

References
1.
Arber W, Linn S. 1969. DNA Modification and Restriction. Annu. Rev. Biochem.. 38(1):467-500. http://dx.doi.org/10.1146/annurev.bi.38.070169.002343
2.
MESELSON M, YUAN R. 1968. DNA Restriction Enzyme from E. coli. Nature. 217(5134):1110-1114. http://dx.doi.org/10.1038/2171110a0
3.
Dussoix D, Arber W. 1962. Host specificity of DNA produced by Escherichia coli. Journal of Molecular Biology. 5(1):37-49. http://dx.doi.org/10.1016/s0022-2836(62)80059-x
4.
Roberts RJ, Murray K. 1976. Restriction Endonuclease. CRC Critical Reviews in Biochemistry. 4(2):123-164. http://dx.doi.org/10.3109/10409237609105456
5.
Kessler C, Manta V. 1990. Specificity of restriction endonucleases and DNA modification methyltransferases ? a review (edition 3). Gene. 92(1-2):1-240. http://dx.doi.org/10.1016/0378-1119(90)90486-b
6.
Roberts RJ. 2003. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. 31(7):1805-1812. http://dx.doi.org/10.1093/nar/gkg274
7.
Pingoud A. 2001. Structure and function of type II restriction endonucleases. 29(18):3705-3727. http://dx.doi.org/10.1093/nar/29.18.3705
8.
Costello JF, Plass C, Cavenee WK. Restriction Landmark Genome Scanning.053-070. http://dx.doi.org/10.1385/1-59259-182-5:053
9.
Bernatzky R. 1989. Restriction fragment length polymorphism.467-484. http://dx.doi.org/10.1007/978-94-009-0951-9_24
10.
Blanc DS, Struelens MJ, Deplano A, De Ryck R, Hauser PM, Petignat C, Francioli P. 2001. Epidemiological Validation of Pulsed-Field Gel Electrophoresis Patterns for Methicillin-Resistant Staphylococcus aureus. Journal of Clinical Microbiology. 39(10):3442-3445. http://dx.doi.org/10.1128/jcm.39.10.3442-3445.2001
11.
Herschleb J, Ananiev G, Schwartz DC. 2007. Pulsed-field gel electrophoresis. Nat Protoc. 2(3):677-684. http://dx.doi.org/10.1038/nprot.2007.94
12.
Velculescu VE, Zhang L, Vogelstein B, Kinzler KW. 1995. Serial Analysis of Gene Expression. Science. 270(5235):484-487. http://dx.doi.org/10.1126/science.270.5235.484
13.
Kuspa A. Restriction Enzyme-Mediated Integration (REMI) Mutagenesis.201-210. http://dx.doi.org/10.1385/1-59745-144-4:201
Restriktsiyalash- restriktaza yoki endonukleaza deb ataluvchi fermentlar yordamida DNK molekulasini spetsifik bo’laklarga bo’lish.
DNK molekulasining 5¹ uchida fosfat, 3¹ uchida gidroksil gruppa bo’ladi. Har bir restriktaza o’zining aniq taniydigan restriktsiya saytiga ega.
Restriktazalar 2 xil kesadi.
1. Yopishqoq uchlar hosil qilib (EcoRI, Hind III);
2. To’mtoqlar uchlar hosil qilib (Hae III).
Restriktsiya mexanizmlarini o’rganishning ahamiyati:

• 
  Restriktsiya mexanizmlarini o’rganish genetik tadqiqotlarni ancha soddalashtirdi.
  
• 
  Restriktsiyalash genetik materialni mayda bo’laklarga bo’lish imkonini berdi.
  
• 
  Fizik usullar (agaroza gelidagi elektroforez) yordamida mazkur bo’laklarni o’lchamiga qarab ajratish, so’ngra har birini restriktsiya xaritalarini tuzish orqali alohida o’rganish mumkin bo’ldi.
  

DNK ni restriktsiyalash genetik injeneriyaning asosi bo’lib, olib borilayotgan genetik tadqiqotlarda keng qo’llaniladi

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