Restriction enzyme

Pulse field gel electrophoresis involves the separation of large DNA fragments, mainly the fragments resulting from digesting a bacterial genome with a rare-cutting restriction enzyme. The unique pattern produced is used to distinguish different strains of bacteria. It may be useful to identify a particular strain as the cause of a widespread disease.^{10, 11}

Serial analysis of gene expression (SAGE) is a technique that involves the quantitative and simultaneous analysis of a large number of transcripts in the form of small tags. Restriction enzymes are used as an anchoring enzyme and a tagging enzyme in this technique.¹²

Restriction enzyme-mediated integration (REMI) involves the use of restriction enzymes to produce compatible cohesive ends in the genome for the insertion of a mixture of plasmid DNA that has been linearized with a restriction enzyme. The plasmid DNA is transformed into the host cell along with a restriction enzyme that facilitates the integration of the DNA into cognate restriction sites in the chromosomes. This technique is useful for genetic screens and for the insertion of genetic and molecular markers at particular points in the genome to identify interesting genes based on their mutant phenotypes.¹³

Materials

Product No.	Description		SDS	Pricing
APAI-RO	Apa Ifrom Acetobacter pasteurianus			Expand