In vitro propagation of lemon verbena: a plant native of South America



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Results 
By the analysis of variance F test at 5% of significance, there was no difference for the dry mass of plants 
and number of leaves. The fresh mass of plants showed a significant difference for the factors culture media 
× sucrose concentrations and sucrose concentrations × agar concentrations. For the number of nodes, there 
was significant difference only for sucrose concentrations. For plant length, there was significance for the 
type of culture medium and the sucrose concentrations as well as for sucrose concentrations × agar 
concentrations. For the number of oxidised leaves, there was a significant difference for the triple 
interaction of factors (culture media, sucrose and agar). 
When compared to culture media × sucrose concentrations, the fresh mass of plant had a significant 
difference only for the MS culture medium, for which concentrations of 30 and 15 g L
-1
were similar (0.207 
and 0.241 g, respectively) and compared to the addition of 45 g L
-1
(0.156 g). Comparing sucrose 
concentrations × culture media, there was a significant difference only at the 15 and 30 g L
-1
concentration, 
in which the MS medium was superior to JADS and WPM (Figure 1A). For the sucrose concentrations × agar 
concentrations, there was a significant difference for 10 g L
-1
agar associated with 15 and 30 g L
-1
sucrose 
(0.204 and 0.192 g, respectively), which was greater than that of 45 g L
-1
(0.134 g). For 12 g L
-1
agar, 30g L
-1
sucrose was higher when compared to the other sucrose concentrations (0.186 g). In contrast, for agar 
concentrations × sucrose concentrations, 15 g L
-1
associated with 8 and 10 g L
-1
of agar were higher (0.180 
and 0.204 g, respectively). The concentration of 45 g L
-1
of sucrose with 8 g L
-1
of agar was superior to that of 
all others (0.186 g) (Figure 1B).



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