1- labоratоry work separation of plants and animal proteins by their solubility The purpose of the work


Separation of salt-soluble proteins from wheat flour



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1-labaratory. Obidjanov Axror

Separation of salt-soluble proteins from wheat flour.

After the separation of water-washed flour (after separation of albumin protein fractions) into the porcelain chamber is diluted and filtered for 2-3 min with 10 mL of 10% NaCl solution.

The filter is washed 2 times with the remaining NaCl solution and removed for further work

Separation of alkaline proteins from wheat flour.

After filtering the flour residue on the filter paper (albumin and globulin protein fractions), grate and filter for 2-3 min in 10 mL of 0.2% NaOH solution. To the resulting filtrate add 1 drop of 0.1n solution of acetic acid. In the precipitate, gluten is formed.



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