Chapter- 4 : Reagents (solution) used in clinical laboratories

Staining………

• Different types of blood cells, bacteria, parasites, and tissues together with their cellular elements can be stained by using appropriate types of stains (differential stains) such as Giemsa stain, Wright stain, Gram stain, Leishman stain, Acid Fast Stain, etc.
• Simple stains are used to reveal the morphology (shape, size and content) of an organism(s) and single dye is utilized for the procedure.

Summary questions

• Describe safe handling of strong acid.
• Describe safe handling of Giemsa stain.
• Explain about standard & staining solutions .
• Explain the use of solutions in medical laboratory

References

• Linne Jean Jergenson, Basic techniques of medical laboratory 4th ed. 2000.
• WHO, Manual of basic techniques for a health laboratory 2000.
• Chees brough M.District Laboratory manual for tropical courtiers, Cambridge Univerity press, 2000 (Vol ).
• Chees brough M.District Laboratory manual for tropical courtiers, Cambridge Univerity press, 2000 (Vol II).
• Seyoum B. Introduction to medical laboratory technology students lecture note series 2002.
• www.CDC.gov

• Next chapter will be on : STERILIZATION AND DISINFECTION