ISSN: 2278-4853 Vol 10, Issue 9, September, 2021 Impact Factor: SJIF 2021 = 7.699
Asian Journal of Multidimensional Research (AJMR)
https://www.tarj.in
409
AJMR
The results presented in the graphs (Fig. 1, 2) show that heat treatment in the preparation of the
fat-and-flour composition can be used to suppress the enzymatic action of the enzymes of the
flour component. This is evidenced by changes in the acid and peroxide numbers of the released
fat during storage of the mixtures.
Since the flour component of the fat-and-flour mixture used, namely, the flour of the germ
product of wheat, is naturally not an absolutely sterile product and is seeded with a specific
microflora, represented mainly by epiphytic and field fungi, then, along with enzymes, this is the
cause of spoilage of the product when storage, as well as a factor that reduces its nutritional and
consumer value. Therefore, the microbiological contamination of the studied compositions was
determined.
Determination of the total number of microorganisms was carried out by a standard method by
inoculation on mesopatamia agar. E. coli bacteria were detected on Endo's medium (Levin's
medium or bismuth-sulfite agar), yeast and fungi - on Sabouraud's medium. The samples under
study were stored at a temperature of 5-10C for 3 months. Their microbiological contamination
and compliance with the requirements of SanPiN 0138-03 were checked monthly [3].
First of all, it should be noted that in the studied fat compositions after incubation for 2 months at
a temperature of 37
0
C on the Endo medium, no groups of E. coli bacteria were found, therefore,
further inoculations were not performed on the Endo medium.
Inoculations on Sabouraud's medium for the detection of fungi and yeast and incubation at a
temperature of 32
0
C for 48 (yeast) and 72 (fungi) hours showed that after 3 months of storage in
all heat-treated samples and after 2 months in compositions without heat treatment with content
of up to 30% flour component, they were not found. This indicates that a high concentration of
anhydrous fat in the fat-and-butter composition, even without heat treatment, can have a
sufficient preservative effect, and heat treatment contributes to better preservation of products
during storage.
When sowing on mesopatamia agar, mainly small and medium colonies were revealed:
monotonous, slimy, and superficial and, in the overwhelming majority, deep. Changes during
storage of the total microbial number in fatty compositions containing 20 and 40% flour of wheat
germ product prepared without heat treatment and with heat treatment are shown in Fig. 3.
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