1- labоratоry work separation of plants and animal proteins by their solubility The purpose of the work

Separation of proteins, soluble in alcohol from wheat flour.
Grind 1gr wheat wheat flour in a porcelain basin and add 5ml 70% ethanol to it. We will cut off the resulting suspension and then we will make an elephant. The precipitates in the sediment are prolamine.
Separation of water-soluble proteins from peas.
This method is similar to extraction of alumina
Separation of salt-soluble proteins from legumes (legulin)

Pea contains leguminous globulin protein. This protein is insoluble in water, but soluble in neutral salts. To extract legulin, pour 20 ml of 10% ammonium sulphate solution into 5gr of bean flour and extract at a thermostat for 20 min at 30⁰C (stirring). The resulting solution is filtered on paper, soaked in saline solution. The resulting filtrate is used to check the globulin protein solubility of pea flour. To do this, add 1 ml of NaCl saturated solution to 1 ml of filter. Filter the filtered leguline and dissolve the filter in 1 ml of 10% NaCl solution. Then we react with the biuretics jet. The results are entered in the table below.

The results of the qualitative analysis of the fractional composition of the protein under investigation.

Scheme 4