1- labоratоry work separation of plants and animal proteins by their solubility The purpose of the work

Separation of salt-soluble proteins of muscle tissues
Squeeze the remaining tissue in the gauze (after the removal of water-soluble proteins), add 10 ml of 10% ammonium sulphate solution, and grind the proteins into a globulin fraction. The extract extracted and filtered for a period of time.

Stroma protein deposition is used for secretion of alkaline protein, which is soluble in protein.

The resulting filtrate is divided into two parts, with a solution of ammonium sulphate saturated with 2ml and half fillings. The globulin filter is filtered through the paper and tested for solubility when it is injected. Biilet jelly is added to the filtrate to see if there are no proteins in the solution.

To precipitate myosin in the second portion of the filter, add 5 ml of globulin to it, then add NaCl powder and heat to full. The resulting sediment consists of fibrillar protein - myosin, which is separated by centrifuge in 5 minutes. We dissolve the myosin at the bottom of the flask by adding distilled water and conduct a qualitative reaction with biuretics reagent.

Separation of alkaline soluble proteins of muscle tissues.
After extraction of water and salt-soluble proteins, we put the precipitate in another tube and place in 5ml 10% NaCl and place in a boiling water bath for 20 min. The resulting solution is cooled and filtered. To 1 ml of 3 ml of filter, 0.1n acetic acid is added to neutralize alkaline. The precipitate is filtered after 5 minutes, with 1 ml of biuretics reagent added to it. The resulting experimental results are summarized in table 1.

Control questions:
1. How Important is Protein for Life?

 Explain it?

2. What types of proteins do you know?

3. What do you know about animal proteins?

4. Why is Biuret jet used?