1- labоratоry work separation of plants and animal proteins by their solubility The purpose of the work

Globulin x and myogenic proteins make up 20-25% of all muscle tissue.

The results of the qualitative analysis of the fractional composition of the protein under investigation.

Verifying material	The solvent	Name of soluble protein	How to get from the solvent	Protein reaction

The chromoproteid myoglobin is red, contains iron, and is red in meat. Myoglobin combines with oxygen to form oximioglobin.

When oxygen is exposed to myoglobin for long periods, brown methmioglobin is formed, so the meat changes from red to brown when stored in the open air for a long time.

Myoglobin denatures at 60⁰C and loses its red color.

Mioalbumin - acetone is easily separated from the plasma by water soluble in water and precipitates with NaCl. Deposits with ammonium sulphate. Mioalbumin and myoglobin make up 1 ... 2% of muscle tissue.

Myosin is an essential salt-soluble protein of muscle tissues, which absorbs water and has a water-retaining properties, making up about 40% of all tissue proteins. Actin is 15%, and it forms actinmiosin, which has a high viscosity along with myosin. The sarcoplasmic protein is part of an incomplete protein, which contains collagen and elastin, which is not an indispensable tryptophanic acidKоllagen va elastining asоsiy miqdоri birlashtiruvchi to’qimalarida bo’ladi.

Miogen (albumin fraction) is the main water-soluble protein of muscle tissues. It is a heterogeneous protein, which is water soluble and precipitates in ammonium oats.

Myosin (globulin fraction) - is the main salt-soluble protein of muscle tissues and represents a fibrillar protein. This protein is soluble in neutral salts and precipitates in a saturated NaCl solution. Pure myosin is well soluble in water. Stromma proteins are an insoluble protein of muscle tissues and are mainly callogenic and elastin.
Completion of work: Separation of water-soluble proteins of muscle tissues.

Place 2 g of homogenized muscle tissue in a 100 ml bottom tube, insert 12 ml of distilled water, and extract for 3 min at a thermostat at 3000C. (mixed). At the same time, albumin fraction of protein is converted to protein (myogenic, myoalbumin, myoglobin, globulin, etc.). At the same time, we dissolve the water-soluble fractions of the muscle tissue 2.3 min and filter through 2 layers of gauze. The extracted filtrate is used to test the solubility of albumin. Water-washed sediment is removed to remove globulin.