1- labоratоry work separation of plants and animal proteins by their solubility The purpose of the work

5. Prоlamins and gliadins. They are 70-80% ethanol-soluble proteins, insoluble in water, salt solutions and pure spirits. Their main representative, gliadin, is found in the endosperm of the wheat grain.

6. Glutelin. These are low-soluble proteins (0.2% NaOH) and are insoluble in neutral solvents.

Completion of work: Separation of water-soluble proteins from wheat flour.

Grind 1gr of wheat flour in a porcelain basin and add 10ml of distilled water. The resulting mixture is paused for 2-3 minutes and passed through an elephant. The remainder of the flour on the ivory tray is washed 2 times with a little distilled water, leaving it to separate the globulin from the wheat. The remaining filtrate is used to check the solubility of albumin proteins.

Add the ammonium sulphate powder powder to the albumin protein fraction and heat it to a temperature not over 40⁰C until full. We pass through the filter of the sediment. Dissolve the precipitate on filter paper with 1 ml of distilled water. We will check if the protein is in the solution and add 1 ml of biuret jet.