“ фаол тадбиркорлик, инновацион ғоялар ва технологияларни қЎллаб-қувватлаш йили”

4. КИМЁ ФАНЛАРИ БЎЛИМИ 
 
 
INVESTIGATION OF THE QUALITY AND QUANTITY ANALYSIS
OF THE CAPSULE OF THE “IODINE-
β-CD” 
Fazliev S.A. – 4th year student, 6/1 group, Faculty of Pharmacy 
Scientific advisers: prof. - S.N. Aminov, D.Sc. - Sharipov A.T. 
Tashkent Pharmaceutical Institute, Tashkent, Department of Inorganic, Analytical
Physical and Colloid Chemistry. 
Relevance of work: The study of the quality characteristics and quantity determination of 
medicines
has a significant importance, because it is obvious that these are the basic requirements for 
preparations in order to be allowed medicine application. Any type of new pharmaceutical is 
certainly experience this stage and the results can surely predict prospects as well as the main 
aspects of the further investigations. Developing novel and convenient methods of the analysis in 
order to increase the specification and efficiency of the experiments is one of the urgent problems 
nowadays. 
Objective: Investigating the quality and quantity characteristics of the capsule of “Iodine-
β-CD” 
Materials and methods: During experiments authors used the mass of the capsule of “Iodine-
β-
CD” developed in Tashkent Pharmaceutical Institute, produced in January of the 2017 year. Has 
been determined various characteristics such as identification, fractional makeup, quantity analysis. 
Ultraviolent spectrophotometric method and high performance liquid chromatography, a string of 
sieves, chemical methods were used to define above mentioned criteria.
UV-spectres were taken to prove identification of the active pharmaceutical substance in the 
preparation. This method is based on the reaction in which all iodine ions with concentric sulphur 
acid, which oxidizes to the molecular iodine and with original iodine initially added to the 
cyclodextrine space gives us the sum of iodine mass. In order to remove impurities and other 
components of the mass of the capsule of “Iodine-
β-CD” we extracted the resulting iodine 
molecules into chloroform. To be ensure that all water and some substances dissolved in it were 
separated we filtered through paper filter provided waterless sodium sulfate, which were expected 
to absorb the water. Obtained organic solution containing iodine molecules were used to take UV-
spectres. A number of experiments were taken place in order to choose suitable solvent and 
wavelength. Using a string of sieves we conducted experiments to determine fractional makeup of 
the mass. Quantity analysis is also based on the reaction used in identification and the optical 
density of the solution served as a measurement of the quantity of the iodine.
Results: On the basis of the UV-spectres it was known that iodine solution in the chloroform has a 
maximum of light absorption in two points, one in 244 nm and the second one in 511 nm. But the 
first one was not so accurate that was unable to use and there were many additional mixed 
maximums. So the sphere in 511 nm was chosen as the key point to identify iodine. For the standard 
pure iodine was used. As for quantity analysis the optic density of the solution in the wavelength of 
the 511 nm used as an analytical signal. For calculation of the iodine content used an equation 
below: 
𝑋𝑋 =
𝐷𝐷
1
∙ 𝑎𝑎
0
∙ 20 ∙ 2 ∙ 𝑃𝑃
𝐷𝐷
0
∙ 25 ∙ 10
 
where, D
0
and D
1
– optic density of the standard and experimental solutions respectively, a
0
– mass 
of the standard iodine, P – the content of the standard.