Improved pFastBac™ donor plasmid vectors for higher protein production using the Bac-to-Bac® baculovirus expression vector system

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Accepted Manuscript
Title: Improved pFastBac
TM
donor plasmid vectors for higher
protein production using the Bac-to-Bac® baculovirus
expression vector system
Authors: Hui Shang, Tyler A. Garretson, C.M. Senthil Kumar,
Robert F. Dieter, Xiao-Wen Cheng
PII:
S0168-1656(17)30678-8
DOI:
http://dx.doi.org/doi:10.1016/j.jbiotec.2017.06.397
Reference:
BIOTEC 7928
To appear in:
Journal of Biotechnology
Received date:
18-1-2017
Revised date:
14-4-2017
Accepted date:
15-6-2017
Please cite this article as: Shang, Hui, Garretson, Tyler A., Senthil Kumar, C.M., Dieter,
Robert F., Cheng, Xiao-Wen, Improved pFastBac
TM
donor plasmid vectors for higher
protein production using the Bac-to-Bac® baculovirus expression vector system.Journal
of Biotechnology http://dx.doi.org/10.1016/j.jbiotec.2017.06.397
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1 
Submitted to Journal of Biotechnology 
 
Improved pFastBac™ donor plasmid vectors for higher protein production using the Bac-
to-Bac

 baculovirus expression vector system 
 
Running title:
Improved baculovirus expression vectors 
 
Hui Shang
1,2*
, Tyler A. Garretson
2*
, C.M. Senthil Kumar
2**
, Robert F. Dieter
2
and Xiao-Wen 
Cheng
1,2 
1
Graduate Program in Cell, Molecular, and Structural Biology, 
2
Department of Microbiology, 
Miami University, Oxford, Ohio 45056 USA 
*Both authors contributed equally to this manuscript 
** Current address: Indian Institute of Spices Research, Marikunnu, P. O., Kozhikode, Kerala, 
India 673012 
Corresponding author: 
Xiao-Wen Cheng, Ph.D. 
Department of Microbiology 
32 Pearson Hall, Miami University, Oxford, Ohio 45056 USA 
Tel #: [phone] 
Fax # [phone] 
Email: [e-mail] 
Highlights 

Bac-to-Bac expresses lower protein levels than wild type AcMNPV 

The transfer vectors of Bac-to-Bac lack an 85 bp cis element and contain SV40 pA 

The polyhedrin pA is required for higher protein expression levels

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