Table haplotype composition (GenBank accession number) for each species. For each site, basin, stream, altitude and geographical coordinates are shown; also site location in Múrria et al. (2013) is given. Species



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Table S1. Haplotype composition (GenBank accession number) for each species. For each site, basin, stream, altitude and geographical coordinates are shown; also site location in Múrria et al. (2013) is given.


Species

GenBank accession number

Basin

Stream

Altitude

Latitude

Longitude

Site

(Múrria et al. 2013)



H. solerorum

JQ687905, KF255657-KF255659

Guadalhorce

Caballos

415

36º40'29"N

04º54'27"W

B52

H. siltalai

HM134823

Llobregat

Llobregat

817

42º14'22"N

01º57'15"E

H05




HM134823, HM134824

Llobregat

Llobregat

760

42º14'11"N

01º53'46"E

H06




HM134823, HM134824

Ter

Ges

606

42º04'50"N

02º18'33"E

H08




HM134829

Ter

Sorreigs

550

42º00'11"N

02º11'46"E

H09




HM134824

Llobregat

Cardener

336

41º52'47"N

01º42'42"E

H11




HM134823, HM134824

Tordera

Arbúcies

335

41º49'04"N

02º29'49"E

H12




HM134823, HM134824, HM134827, HM134828

Besós

Vallcàrquera

376

41º43'41"N

02º16'58"E

H13




HM134823, HM134825, HM134824

Besós

Cànoves

420

41º42'34"N

02º20'55"E

H14




JQ687902

Jara

Arquillo

990

38º45'10"N

02º21'45"W

T26




JQ687902

Segura

Mundo

806

38º30'30"N

02º14'54"W

T27




KF255651, KF255652

Genil

Juntas

860

37º25'53"N

03º39'08"W

B39




KF255651, KF255654

Genil

Colomera

780

37º24'08"N

03º43'11"W

B40




HM134823, KF255651, KF255652, KF255653

Guadiana Menor

Gor

1333

37º20'53"N

02º56'55"W

B41




KF255649, KF255650

Miel

Miel

220

36º06'42"N

05º30'19"W

B53

H. infernalis

JQ687904

Genil

Genil

1060

37º09'05"N

03º24'50"W

B43




JQ687904

Guadiana Menor

Tejos

1660

37º07'07"N

03º4'56"W

B44




JQ687903

Genil

Cerezal

1060

36º55'36"N

03º59'11"W

B48




JQ687904

Genil

Añales

970

36º55'02"N

03º53'32"W

B49

H. fezana

HM134821

Laou

Laou

560

35º18'42"N

05º21'12"W

R56




HM134821

Laou

Laou

400

35º14'21"N

05º10'32"W

R59




HM134821, HM134822

Laou

Laou

905

35º06'48"N

05º11'08"W

R61

H. fontinalis

JQ687908

Guadiana Menor

Guardal

1090

37º53'51"N

02º40'31"W

T35

H. cf. spiritoi

KF255655

Foix

Pontons

540

41º25'12"N

01º30'30"E

H16




KF255655

Foix

Pontons

660

41º25'01"N

01º31'23"E

H17




KF255655, KF255656, JQ687906

Ebre

Canaletes

720

40º54'19"N

00º21'32"E

H20

H. instabilis

JQ687922

Llobregat

Llobregat

817

42º14'22"N

01º57'15"E

H05




KF255635, KF255637

Ter

Ges

606

42º04'50"N

02º18'33"E

H08




JQ687922

Tordera

Arbúcies

335

41º49'04"N

02º29'49"E

H12




KF255637

Tordera

Arbúcies

335

41º49'04"N

02º29'49"E

H12




JQ687922

Besós

Cànoves

420

41º42'34"N

02º20'55"E

H14




KF255634, KF255636

Ebre

Canaletes

720

40º55'26"N

00º21'29"E

H20




JQ687921

Guadalfeo

Trevélez

1860

37º00'16"N

03º28'38"W

B46




JQ687921

Genil

Cerezal

1060

36º55'36"N

03º59'11"W

B48

H. cf. spiritoi

JQ687906

Llobregat

Marganell

321

41º38'21"N

01º47'12"E







JQ687906

Leza/Ebro

Tejada

882

42º12'33"N

02º30'17"W




H. spiritoi

Hspi1, Hspi2 (KY678901, KY678902)

Mandre

Villadoro

660

37º42'43"N

14º17'51"E







Hspi3 (KY678903)

Argens

Bresque

376

43º34'57"N

05º08'60"E







Hspi3 (KY678903)

Durance/Rhône

Calavon

500

43º57'48"N

05º36'22"E







Hspi3 (KY678903)

Durance/Rhône

Calavon

467

43º56'58"N

05º35'51"E







Hspi3 (KY678903)

Ouvèze/Rhône

Toulourenc

582

44º10'30"N

05º25'60"E




H. ambigua

HapC11 (KY656176)

Deva

Corvera

550

43º14'16"N

04º38'33"W







HapC19, HapC42 (KY656184,KY656207)

Nansa

Venta Fresnedo

193

43º16'47"N

04º29'31"W







HapC36 (KY656201)

Sella

Covadonga

137

43º18'53"N

05º04'18"W







HapC39 (KY656204)

Cares

Duje

855

43º13'36"N

04º45'22"W




H. tenuis

HapC33 (KY656198)

Ain

Hérisson

785

46º36'33"N

05º53'01"E






Table S2. Complete locality information of individuals morphologically described. For each site, basin, stream and/or locality, altitude and geographical coordinates are shown.


Species

Basin

Stream/Locality

Altitude

Latitude

Longitude

H. solerorum

Guadalhorce

Caballos/Tolox

415

36º40'31''N

04º54'27''O




Guadalhorce

Caballos/Tolox

340

36º40'50''N

04º54'31''O




Guadalhorce

Horcajos/Tolox

472

36º41'36''N

04º56'36''O




Guadalhorce

Pereilas/Coín

206

36º39'16''N

04º47'15''O




Guadalhorce

Corcho/Coín

428

36º36'37''N

04º47'33''O

H. cf. spiritoi

Llobregat

Coaner/Coaner

630

41º50'04''N

01º43'25''E




Foix

Pontons/Pontons

660

41º25'04''N

01º31'22''E




Foix

L’Albareda/Fontrubí

488

41º25'21''N

01º35'36''E




Llobregat

Cornet/Sant Salvador de Guardiola

240

41º41'35''N

01º47'47''E




Llobregat

Vilanova/Avinyó

470

41º54'30''N

01º58'26''E




Llobregat

Sant Cugat/Sant Cugat del Racó

310

41º48'06''N

01º48'13''E




Llobregat

Can Relat/Sentforas

567

41º54'25''N

02º12'35''E




Llobregat

Gaià/Gaià

456

41º55'17''N

01º55'59''E




Llobregat

Marganell/Marganell

321

41º38'23''N

01º47'12''E




Aragón/Ebro

Bailo

701

42º30'35''N

00º48'46''O




Mijares

Fuen Lozana/Mora de Rubielos

1037

40º15'10''N

00º45'17''O




Jalón/Ebro

Piedra/Monasterio de Piedra

741

41º11'40''N

01º47'13''O




Leza/Ebro

Tejada/Jalón de Cameros

882

42º12'36''N

02º30'17''O




Segre

Alinyà/Alinyà 

931

42º10'48''N

01º25'24''E

H. spiritoi

Durance/Rhône

Calavon/Vailsaintes

500

43º57'51''N

05º36'23''E




Durance/Rhône

Calavon/Simiane la Rotonde

467

43º56'59''N

05º35'51''E




Ouvèze/Rhône

Toulourenc/Montbrun les Bains

582

44º10'33''N

05º26'00''E


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