SylbV01-00-00. pmd

Unit 2:
Mapping and sequencing of mulberry and silkworm
Mapping and sequencing of mulberry and silkworm. Genome of
mulberry and silkworm. Molecular basis for improvement of yield
components in mulberry and silkworm.
BLOCK 2: Tissue culture and Recombinant DNA techniques
Unit 1:
Tissue culture in mulberry
Micro propagation in mulberry, Production of haploids and Double
haploids (DH) lines, Synthetic seeds, Induction of 
in-vitro 
flowering,
In-vitro
screening of mulberry for different stress conditions.
Somaclonal and Gametoclonal variations - their scope and
applications.Cryopreservation in mulberry for germplasm preservation.
Protoplast culture and somatic hybridization.
Unit 2:
Recombinant DNA techniques in mulberry and silkworm
Recombinant DNA techniques in mulberry and silkworm. Role of agents
and microorganisms with emphasis to common vectors for gene transfer.
Stability and expression of transferred genes in mulberry and silkworm.
Germline transformation and scope of genetic manipulation between
silkworm breeds. Application of molecular techniques in gene
identification for further breeding programmes. Application of site
directed mutagenesis, gene targeting and gene therapy. Silkgland
genetics.
Units 3:
Seri bioinformatics
Seri bioinformatics- introduction, branches of bioinformatics, computer
programmes used. application in crop improvement. Studies on
Genomics- genomics in crop improvement, types of genomics: structural,
functional and applications, achievements and limitations. Studies on
proteomics, Metabolomics. Nano technology- introduction, main
features, Application of nano technology in mulberry improvement.
VII. Practical
• RFLP marker technique as applied to mulberry crop;
• RFLP marker technique as applied to silkworm improvement programmes;
• RAPD marker technique as applied to mulberry crop;
• AFLP marker technique as applied to mulberry crop;
• SSR marker technique as applied to mulberry crop;

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Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
796
• Equipments and chemicals used in RFLP and RAPD techniques;
• Equipments and chemicals used in PCR technique;
• Hands on training in mulberry DNA extraction, isolation, purification and
concentration;
• Hands on training in silkworm DNA extraction, isolation, purification and
concentration;
• DNA quantification and quality assessment in mulberry;
• DNA quantification and quality assessment in silkworm;
• Procedure of Agarose gel electrophoresis;
• Application of PAGE in silkworm;
• PCR reaction; PCR amplification;
• Estimation of genetics distances- cluster analysis in mulberry;
• Estimation of genetics distances- cluster analysis in silkworm;
• Visit to Seribiotech. Lab. of CSB at Kodathi;
• Visit to MAS lab and biotechnology lab of UAS (B);
• Visit to CSR&TI, Mysore- biotechnology division.