SylbV01-00-00. pmd

VII. Practicals
• Laboratory safety rules;
• Seri biotechnology lab and its facilities;
• Preparation of MS medium for tissue culture in mulberry;
• Selection, collection and preparation of plant material for mulberry tissue culture;
• Culturing of plant material/explant in culture media;
• Tissue culture techniques for mulberry propagation;
• Hardening of tissue cultured mulberry plants;
• Isolation of genomic DNA- mulberry leaf;
• Isolation of genomic DNA- mulberry leaf;
• Amplification of DNA in mulberry by using PCR;
• Study of diversity of mulberry germplasm by using molecular markers;
• Study of diversity of mulberry germplasm by using molecular markers;
• Comparative study of diversity of mulberry germplasm through morphological traits
and molecular markers;
• Techniques for gene transformation in mulberry;
• Techniques for gene transformation in mulberry;
• Molecular databases in mulberry;

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Sericulture
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• Visit to MAS lab in Department of Biotechnolgy, UAS, GKVK, Bengaluru;
• Visit to Seri-Biotechnology Research Laboratory, CSB, Kodathi, Bengaluru.
VIII. Teaching Methods/ Activities
– Lectures
– Assignments (Reading/ Writing)
– Text books/ Publication reviews
– Student presentations
– Group work
– Student’s interview of key policy makers
– Case analysis and case studies and guest lectures
– Review of policy documents
– Visits
IX. References
Burrell MM. 1993. 
Enzymes of Molecular Biology
. Humana Press Inc., NewYork.
Kendrew J. 1994. 
The Encyclopedia of Molecular Biology
. Blackwell Scientific Publications,
Oxford.
Old RW and Primrose SB. 1994. 
Principles of gene manipulation: An Introduction to genetic
engineering
, 5
th
Ed. 
Blackwell Scientific Publications
, U. S. A.
Hansen G and Wright MS. 1999. Recent advances in the transformation of plants. 
Trends in
Biotech., 
13: 
324-331.
Vijayan K. 2004. Genetic relationship of Japanese and Indian mulberry (
Morus
spp.) genotypes
as revealed by DNA fingerprinting. 
Plant Syst. Evol.,