SylbV01-00-00. pmd

Unit 2:
Mulberry germplasm characterization
Mulberry germplasm characterization by using molecular markers.
Introduction, features of ideal DNA markers, types of DNA markers,
uses in crop improvement. Application of biotechnological tools in
screening for biotic and abiotic stress tolerance in mulberry.
Unit 3:
Genotyping and phenotyping
Introduction, definition of genotype and phenotype. Phenotyping-
advantages and disadvantages. Methods of genotyping, advantages
and disadvantages of genotyping and applications of genotyping. Marker
Aided Selection (MAS) for economically important traits in mulberry.
Steps involved in MAS. Application of MAS, advantages of MAS,
limitations of MAS.

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Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
760
BLOCK 2: Recombinant DNA technology
Unit 1
:
Genes transfer systems
Vector mediated gene transfer, microinjection, electroporation, direct
DNA uptake, gene gun technique, selectable markers and reporter
system; comparison of transgenic technology and traditional breeding
methods, detection of transgenic mulberry. Prospects of transgenic
mulberry. Procedure of development of transgenic mulberry.
Advantages and disadvantages of transgenic mulberry. Risks in
transgenic technology. Biosafety and regulatory issues, Achievements.
Unit 2:
QTL mapping
Development of maps, Advantages and limitations of QTL mapping.
Methods of mapping. Requirements and steps involved in QTL mapping.
Mapping populations (F2S and back crosses RILs, NILs, DHs). Tagging
of economically important traits in mulberry.
Unit 3
:
Seri bioinformatics
Bioinformatics in crop improvement-introduction, branches of
bioinformatics, computer programmes used in biology, applications in
crop improvement, varietal information system, PGR data base. Studies
on Genomics- genomics in crop improvement, types of genomics:
structural, functional and applications, achievements and limitations.
Studies on proteomics. Studies on metabolomics, advantages of
bioinformatics, limitations. Intellectual Property Rights. Plant variety
protection act (PVPA): introduction, types of protection, basic
requirements, organizations involved, procedure of PVP, material to
be protected, types of varieties, exemptions under PVPA, advantages
and disadvantages of PVPA. Nano- technology: introduction, main
features, Application of nano-technology, application in mulberry
improvement.