SylbV01-00-00. pmd

I. Course Title
: Genomics in PGR management
II. Course Code
: PGR 508
III. Credit Hours
: 2 (1+1)
IV. Why this course ?
In addition to conventional hybridization, there is a need for precise tools to decipher
the molecular basis of genetic diversity through mapping and sequencing.
V. Aim of the course
Students would be taught basics of genome structure and organization, generation
of molecular markers-basic principles, molecular marker techniques, data handling
and analysis of GM.
VI. Theory
Unit I
Structure and function of DNA, genome organization, tools and techniques for
genetic manipulation, Introduction to genetic markers, classification and comparison
of markers, basis for DNA polymorphism and principles of generating molecular
markers- RFLP, PCR, sequencing, next generation sequencing techniques, molecular
marker techniques eg. RAPD, ISSR, AFLP, etc.; STMS, SNPs markers, GBS, GWAS,
data handling and statistical analysis.
Unit II
Overview of molecular marker applications and recent advances, genetic diversity

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Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
analysis using molecular markers, DNA Fingerprinting and cultivar identification.
Unit III
Introduction to transgenics, development of genetically modified crops, monitoring
strategies and methods for detecting transgenics, Genome Editing.
VII. Practical
• DNA isolation and purification, DNA quantification;
• RAPD, ISSR, STMS, SCAR, SRAP;
• Data Analysis.
VIII. Teaching methods
• Lectures
• Power point presentations
• assignments, quiz
• Group tasks, student’s presentations
IX. Learning Outcome
Knowledge on current state-of-the-art technological developments of genomics era,
current challenges being faced and handled.