SylbV01-00-00. pmd

Unit 2.
Non-conventional methods of breeding
Present status of mulberry varietal improvement through mutation.
Importance of induced mutation, recent achievements in mulberry
mutation breeding. Limitations of mutation breeding.
Polyploidy, induction of polyploidy in mulberry, special features of
triploids in mulberry, process of triploid mulberry development,
varieties developed by polyploidy breeding in mulberry. Breeding
methods followed for leaf quality parameters, biotic and abiotic stress.
Breeding strategies for climate change. Participatory plant breeding
(PPB) – introduction, types, stages of participation, objectives,
advantages of PPB, role of farmers in PPB.
Unit 3:
Biotechnological approaches for mulberry improvement
Recent advances in application of plant tissue culture. Applications of
molecular markers in mulberry improvement. Genome characterization.

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Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
776
Development of transgenic mulberry – procedure. Nanotechnology:
introduction, main features and its applications. Plant Variety
Protection Act (PVPA) – Introduction, types of protection, basic
requirements, organizations, procedure, material to be protected, types
of varieties, exemptions under PVPA, advantages and disadvantages
of PVPA. Statistical approaches for yield tests in mulberry: Field plot
techniques in mulberry breeding experiments. Different experimental
designs-RCBD, Augmented Randomized Complete Block Design
(ARCBD) and LSD.
VII. Practicals
• Geographic distribution of the genus 
Morus, 
using maps;
• Evaluation of mulberry germplasm maintained at the Department of Sericulture,
UAS, GKVK, Bengaluru;
• Study of diversity of mulberry germplasm maintained at the Department of
Sericulture, UAS, GKVK, Bengaluru;
• Collection and categorization of available mulberry germplasm using standard
key;
• Studies on conservation and maintenance of mulberry Gene bank;
• Identification of suitable mulberry genotypes for tree mulberry;
• Characterization of suitable mulberry genotypes and quality parameters for chawki
silkworm;
• Characterization of suitable mulberry genotypes and quality parameters for late
age silkworm;
• Identification of suitable mulberry genotypes for fruit purpose;
• Evaluation of commercially released mulberry varieties for growth and yield
parameters;
• Phenotypic evaluation of commercially released mulberry varieties;
• Hands on training in callusing, sub-culturing,root initiation, shoot initiation and
hardening of tissue culture plants, Triploids, etc.;
• Active bud treatment for polyploid induction in mulberry;
• Layout of field experiments in mulberry;
• Testing for resistance to biotic stresses;
• Testing for resistance to abiotic stresses;
• Selective breeding using marker assisted selection for identifying WUE mulberry
genotypes;
• Visit to CSGRC Hosur/ CSB.