SylbV01-00-00. pmd

Unit II
DNA sequencing. Structural genomics. Functional genomics. Transcriptomics.
Proteomics. Metabolomics.
Unit III
Recombinant DNA Technology, Transgenics, Vectors,. Gene transfer strategies –
direct and indirect. Molecular characterization of transformants. Application of
transgenics in forestry.
VI. Practical
• Isolation of DNA, RNA from forest tree species;
• Isozyme analysis;
• Use of molecular markers and RAPD and RFLP for clonal identification;
•
Agrobacterium
mediated gene transfer;
• Preparation of linkage maps.
VII. Suggested Reading
Brown CM, Campbell I and Preist FG. 2005. 
Introduction to Biotechnology
. Panama Publishers.
Chawla HS. 2004. 
Introduction to Plant Biotechnology
. Kalyani Publishers.
Kole C 2007. 
Genome Mapping and Molecular Breeding in Plants
. Springer.
Schnell RJ and Pridarshan PM. 2012. 
Genomics of Tree Crops
. Springer.
Singh BD. 2006. 
Biotechnology – Expanding Horizons
. Kalyani Publishers.
Lecture Schedule
Sr. No.
Topic
No. of Lecture (s)
Theory
1.
Biochemical markers (Isozymes and Monoterpenes)
2
2.
Molecular markers – Non-PCR based (RFLP) and PCR based (RAPD,
ISSR, SSR, AFLP, SNP, etc.)
5
3.
Application in forestry – quantification of genetic diversity
2
4.
Marker assisted selection. Genetic maps of selected forest trees
4
5.
DNA sequencing
2
6.
Structural genomics, functional genomics
4
7.
Transcriptomics, proteomics, metabolomics
4
8.
Recombinant DNA Technology, Transgenics, Vectors
4

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Forestry–Forest Biology and Tree Improvement
611
9.
Gene transfer strategies – direct and indirect
3
10.
Molecular characterization of transformants. Application of transgenics
in forestry
2
Total
32
Practical
1.
Isozyme analysis
2
2.
Isolation of DNA, RNA from forest tree species
2
3.
Use of molecular markers and RAPD, SSR and RFLP for clonal
identification
6
4.
Agrobacterium
mediated gene transfer
4
5.
Preparation of linkage maps
2
Total
16