SylbV01-00-00. pmd

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I. Course Title
: Genomic Tools and Current Applications
II. Course Code
: PGR 608
III. Credit Hours
: 3(2+1)
IV. Why this course ?
Plant genomics aims to develop high-throughput genome-wide-scale technologies,
tools and methodologies to elucidate the basics of genetic traits/ genetic diversity
in organisms
V. Aim of the course
To provide knowledge in genomic tools and their application in plant genetic resource
exploration, collection, conservation and utilization.
VI. Theory
Unit I
Genomics: Basic concept, structural, comparative and functional genomics, genomic
tools, TILLING, EcoTILLING, Genome duplication and ploidy variation, application
of genomic tools in PGR valuation, conservation and utilization.
Unit II
DNA barcoding: Basic concept, methodologies, utility of DNA barcoding in species
delineation, plant exploration and collection, conservation and utilizing species in
widening gene pool of major crops.
Unit III
DNA markers: Types, application in assessment of diversity in germplasm, DNA
fingerprinting and genetic identity analysis, allele mining, development and
validation of core sets, genetic association studies and genomic selection in
germplasm collections.
VII. Practical
• Gene based screening of trait-specific germplasm using linked molecular markers;
• Amplification and sequencing of DNA barcoding loci for species identification in
crops;
• Allele mining in trait-specific germplasm for quality traits in crops;
• DNA fingerprinting for identity analysis in crops, molecular markers for designation
and validation of germplasm core-sets.
VIII. Teaching methods
• Lectures
• Power point presentations
• Assignments, quiz
• Group tasks, student’s presentations