SylbV01-00-00. pmd

VII. Practical
• Collection and evaluation of germination of seed lots with different vigour status;
• Evaluation of seed vigour by physical vigour test – seed size, colour, weight –
turbidity test;
• Evaluation of seed vigour by physiological vigour test – imbibition pattern, speed
of emergence, radicle emergence, germination, seedling measurement and
computation of various index;
• Conducting different stress tests – brick gravel and paper piercing tests;
• Conducting accelerated ageing and controlled deterioration test;
• Conducting chemical stress test – NH
4
Cl, NaCl, mannitol, PEG test;
• Special vigour tests – cool germination test – cold test – anaerobic test;
• Biochemical vigour test – electrical conductivity, free sugars and amino acid test
in seed leachate;
• Estimation of dehydrogenase enzyme activity;
• Estimation of free fatty acids in seed lots in varying vigour levels;
• Bio-assay test for seed vigour;
• Estimation of volatile aldehydes in different crop seeds with varying vigour;
• Correlation studies between field emergence and different vigour tests;
• Seed vigour on field establishment, population maintenance and crop growth and
productivity;
• Pre-sowing vigour management techniques;
• Pre-storage and mid storage vigour management techniques.
VIII. Teaching methods
• Classroom lectures
• Assignment and presentation
• Slides/ video shows

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102
Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
• Practical exercise
• Hands on training
IX. Learning outcome
This course will enable the students to understand the concept of seed vigour and
enhance the analytical skills to predict and assess the vigour accurately so as to
adjust the seed lots for its value for cultivation and usage.