Insert the plug in mains socket and switch the power ON .Allow the instrument to warm up for about 5min.
1)Zero Adjustment: Rotate slowly the wavelength selector knob. Insert the test tube filled with solvent in its place. Adjust the "Zero adjustment knob till the pointer comes to zero.( zero reading of transmittance).
2)100% Adjustment:Rotate slowly the wavelength selector knob till desired wavelength is selected. Do not disturb the filled test tube. Adjust the "100%" knob till the pointer comes to 100.
Repeat steps 1 & 2 above.
The instrument is now calibrated for use.
SOP FOR CONDUCTOMETER
Switch the instrument on about 10-15 minutes before starting the experiment.
Keep the conductivity cell dipped in distilled water for about 10-15 minutes. Connect its terminals to the cell terminals seen on the conductivity meter.
Confirm that the instrument has been properly standardized.
Take the sample solution whose conductance is to be measured, in a 100cm3 beaker.
Remove the conductivity cell from distilled water. Wipe it clean gently with a filter paper and dip it into the sample solution.
Wait for about one minute. Check that the standard switch is kept at up position.
Rotate slowly the range selector knob till the display shows maximum number of significant figures.
Note down the reading shown in the display with an exponent of (-3).This is the conductance of the sample.
Switch on the instrument and let it to warm up for about 10 to 15 minutes.
Switch on to standardization mode.
Now display must read standard potential1.018V.
If it does not read so, use screw driver and adjust it to 1.018 V.
SOP FOR pH METER
Switch on the instrument and let it to warm up for about 10 to 15 minutes.
Adjust the temperature knob to room temperature.
Rinse the electrode with distilled water before dipping in different solution and handle it carefully as it is fragile.
Standardization of the instrument can be done by using the following buffers of pH = 4.00, 7.00 and 9.2.
First adjust buffer of pH= 4.00 followed by 9.2 any variation in pH of 4.00 and 9.2 value can be adjusted using slope knob. Intercept knob is used to adjust a buffer value of 7.00.
Once it is standardized do not disturb the slope/intercept knob.
SOP FOR DIGITAL FLAME PHOTOMETER (DFP)CL- 410 The DFP consists of two units: 1. Main Unit (MU) & 2. Compressor Unit.(CU)
Operating Instructions : Connect the gas tube from LPG cylinder to a fuel gas inlet located on LHS of MU
Connect the air tube from compressor to automiser of MU.
Connect CU to 230V electric main, switch on the electric supply and set the o/p pressure to 0.5 kg/cm2, using the knob provided alongside.
Connect a pipe at the base of MU for the drainage. The other end of the pipe may be kept in a
plastic mug.
Open (rotate anticlockwise)the Fuel Gas Valve (FGV) of the MU by approximately half turn.
Switch on the LPG cylinder regulator and immediately ignite the flame through the ignition window.
Watch the flame through the flame view window. Do the fine adjustments of fuel flow with the help of FGV to get a stable flame having well defined cones.
Connect MU to electric main & switch on the power supply to make the digital display on.
Turn the Set FS knobs (both coarse & fine) as well as Set zero knobs (both coarse &fine) to maximum clockwise position and sensitivity switch to high position.
Select the appropriate filter ( Na or K) with the help of filter selector of the MU.
Feed distilled water to the atomizer and wait for at least 30 sec.
Rotate Set zero (coarse) knobslowly till readout of the digital display approaches to zero. Adjust Set zero (fine) knob to achieve precise zero setting.
Replace D/W with the standard solution of highest ppm and adjust the full scale deflection to 100, with the help of Set FS coarse & fine knobs as well sensitivity switch.
The standard solutions of lower concentrations are then introduced in turn, and their respective emission intensities are recorded. Note that in between any two solutions, D/W must be aspirated and zero background emission intensity must be checked.
Unknown solution is finally introduced and its emission intensity is recorded.
After the completion of estimation, feed the distilled water to the atomizer & aspire it for about 2 minutes to wash out remaining from the mixing chamber, atomizer etc.
Switching off the instrument:
First close the gas cylinder regulator.
After the flame goes off, close the FGV (turn clockwise to maximum) of the MU.
Switch off the power supply to MU.
About two minute later, switch off the power supply to CU.
