Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by hplc-dad

Results and Discussion 
Method Validation 
Linearity 
The regression equation for lycorine was 
y 
= 15. 8423032
x
−
1.339608, and 
for galanthamine, it was 
y
= 11.451763
x
−
2.40657. Excellent linearity was 
obtained for lycorine (
r
2
= 0.99985) and galanthamine (
r
2
= 0.9999) exhibiting 
a good correlation between the alkaloid concentration and the peak area. 
Moreover,
standard deviation values of the slope and the intercept for ly-
corine were found as 0.167 and 8.421, and for galanthamine, 0.314 and 0.382, 
respectively. Standard error of the regression for lycorine was found as 
28.330 whereas, for galanthamine, it was calculated as 18.956. 
F
-factors of 
the calibration curves of lycorine and galanthamine were 0.971 and 0.927, 
respectively. 
Precision 
Intra-day and inter-day variations were examined to determine the preci-
sion of the method. Variations were expressed by relative standard devia-
tion (RSD) for intra- and inter-day. These values were found to be less than 
1.0% for the two compounds indicating the good reproducibility of the 
method. The results are shown in 
Tables II 
and 
III
.
Table II
. Precision of lycorine in 
G. woronowii 
Amount 
(
μ
g mL
−
1
) 
Intra-day precision 
(RSD, %) 
Inter-day precision 
(RSD, %) 
1 0.252 
0.378 
2.5 
0.122 
0.098 
5 0.345 
0.128 
10 0.023 
0.139 
25 0.037 
0.005 
50 0.048 
0.112 
100 0.034 
0.039 
200 0.028 
0.032 
300 0.014 
0.056 

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A. Emir et al.
 
760
Table III. 
Precision of galanthamine in 
G. woronowii
Amount 
(
μ
g mL
−
1
) 
Intra-day precision 
(RSD, %) 
Inter-day precision 
(RSD, %) 
1 0.095 
0.495 
5 0.040 
0.589 
10 0.020 
0.107 
25 0.051 
0.027 
50 0.122 
0.511 
100 0.467 
0.051 
200 0.251 
0.149 
300 0.094 
0.122 
400 0.080 
0.080