Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by hplc-dad

Experimental 
Plant Material 
Specimens of 
G. woronowii
were collected from seven different localities in 
Northeastern Turkey, during flowering time. The plants were identified by 
Prof. M. Ali Onur from the Department of Pharmacognosy, Faculty of 
Pharmacy, Ege University, Izmir, Turkey. Voucher samples of 
G. woronowii
Table I.
G. woronowii
specimens employed in the study 
Sample ID 
Part used 
Collection site 
Voucher sample 
GW-1
B
Bulbus 
GW-1
H
Herba 
Çaykara, Trabzon 
1358 
GW-2
B
Bulbus 
GW-2
H
Herba 
Arhavi, Artvin 
1375 
GW-3
B
Bulbus 
GW-3
H
Herba 
Aral
ı
k village, Borçka,
Artvin 
1392 
GW-4
B
Bulbus 
GW-4
H
Herba 
Near Aral
ı
k village, Borçka, 
Artvin 
1393 
GW-5
B
Bulbus 
GW-5
H
Herba 
Borçka, Artvin 
1414 
GW-6
B
Bulbus 
GW-6
H
Herba 
Kemalpa
ş
a, Hopa, Artvin 
1415 
GW-7
B
Bulbus 
GW-7
H
Herba 
Derepazar
ı
, Rize 
1417 

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Simultaneous Determination of Lycorine and Galanthamine
757 
specimens are deposited in the Herbarium of the Department of Pharma-
cognosy, Faculty of Pharmacy, Ege University. A detailed list of these 
specimens and their collection sites are found in 
Table I
. 
Chemicals and Solvents 
Standard samples of lycorine and galanthamine were previously isolated in 
our laboratory and authenticated by spectral analysis (UV, IR, 1D NMR, 
MS) [21]. TFA (trifluoroacetic acid) (Merck), HPLC grade acetonitrile (Lab-
Scan Analytical Sciences), and chromatographic grade double-distilled wa-
ter were used for the HPLC analysis of the analytes. Other chemicals were 
of analytical grade.