Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by hplc-dad

Limits of Detection (LOD) and Quantification (LOQ) 
The LOD and LOQ were calculated as 0.0492 
μ
g mL
−
1
and 0.1641 
μ
g mL
−
1
for lycorine, respectively. For galanthamine, the LOD was found to be 
0.00745, and the LOQ was determined as 0.0279. 
Recovery 
Recovery assay was carried out by spiking three different known concentra-
tions of lycorine and galanthamine into sample solutions. The mean extrac-
tion recovery of lycorine was in the range of 98.5704–108.3902% whereas, 
for galanthamine, the mean extraction recovery laid between 93.2038 and 
119.3738%. The results of the experiments are given in 
Table IV
.
Table IV.
Recoveries for the determination of lycorine and galanthamine in 
G. woronowii 
Analyte 
Concentration 
in sample 
(mg mL
−
1
) 
Amount 
spiked 
(mg mL
−
1
) 
Mean 
amount 
found 
(mg mL
−
1
) 
Mean recovery 
(%) ± SD 
RSD 
(%) 
0.03 
0.048 
108.390 ± 1.066 
0.983 
0.06 0.058 
98.573 
±
 
1.087 
1.102 
Lycorine 0.058 
0.12 0.092 
103.210 
±
 
1.047 
1.014 
0.075 0.131 
119.374 
±
 
0.502 
0.421 
0.15 0.144 
93.204 
±
 
1.848 
1.983 
Galan-
thamine 
0.159 
0.30 0.231 
100.56 
±
 
2.837 2.822 

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Simultaneous Determination of Lycorine and Galanthamine
761 
Specificity 
Peak purities of lycorine and galanthamine were evaluated by the acquisi-
tion of UV spectra with the DAD detector. The spectra of the analyzed com-
pounds are shown in 
Figs 1
and
2
. In the analyzed extracts, the spectra of the 
compounds were in good agreement with the spectra of the standards. The 
values of spectra match for lycorine and galanthamine were found to be 
999.775 and 999.982, respectively.
Fig. 1
. Spectrum of lycorine 
Fig. 2
. Spectrum of galanthamine
 

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A. Emir et al.