Systemic lupus erythematosus and rheumatoid arthritis



Download 1,18 Mb.
Pdf ko'rish
bet21/44
Sana04.02.2022
Hajmi1,18 Mb.
#430882
1   ...   17   18   19   20   21   22   23   24   ...   44
Bog'liq
FULLTEXT01 2

Paper IV 
Paper V 
pre-patients controls 
patients 
HLA-SE 
50/90 (55.6) 
66/173 (38.2) 
423/735 (57.6) 
ACPA 
33/89 (37.1) 
5/353 (1.4) 
519/759 (68.4) 
IgA-RF 
25/59 (42.4) 
12/236 (5.1) 

IgG-RF 
10/59 (16.9) 
10/236 (4.2) 

IgM-RF 
13/59 (22.0) 
14/236 (5.9) 
568/760 (74.7) 
 
 
12. Functional study (paper V) 
For the functional study, individuals were sampled on the basis of carrying or 
not carrying the 
PTPN22
1858 T risk allele. Blood samples were collected from 
12 patients with RA, 12 SLE patients and 12 healthy controls. The patients were 
age and gender matched with the controls and within the patient groups the six 
PTPN22
1858 CC individuals were gender and age matched with the six 
PTPN22
1858 CT individuals. The blood samples were collected into 
Vacutainer cell preparation tubes (BD biosciences) and monocytes separated by 
centrifugation. T-lymphocytes were purified by immunodepletion of non-T-
cells using Pan T-cell isolation kit II human (Miltenyi Biotec). Cells were 
stimulated with 10 ng/ml phorbol 12-myristate, 13-acetate (PMA) plus 1 µM 
ionomycine; beads coated with monoclonal antibodies to CD3
ε
and CD28 
(Dynal Inc.); or with complete medium alone (RPMI 1640 + Glutamax and 10% 
heat inactivated FCS, 7.5% sodium bicarbonate, Penicillin / Streptavidin (10000 
u/ml / 10000 mg/ml), 100 mM sodium pyruvate and monothioglycerol). The 
cells were activated at a concentration of 10
6
cells/ml for 20h at 37
o
C (5% CO
2
). 
After activation, IL-2 concentration was measured in culture supernatants using 
Human IL-2 ELISA MAX Set Deluxe (BioLegend).

Download 1,18 Mb.

Do'stlaringiz bilan baham:
1   ...   17   18   19   20   21   22   23   24   ...   44




Ma'lumotlar bazasi mualliflik huquqi bilan himoyalangan ©hozir.org 2024
ma'muriyatiga murojaat qiling

kiriting | ro'yxatdan o'tish
    Bosh sahifa
юртда тантана
Боғда битган
Бугун юртда
Эшитганлар жилманглар
Эшитмадим деманглар
битган бодомлар
Yangiariq tumani
qitish marakazi
Raqamli texnologiyalar
ilishida muhokamadan
tasdiqqa tavsiya
tavsiya etilgan
iqtisodiyot kafedrasi
steiermarkischen landesregierung
asarlaringizni yuboring
o'zingizning asarlaringizni
Iltimos faqat
faqat o'zingizning
steierm rkischen
landesregierung fachabteilung
rkischen landesregierung
hamshira loyihasi
loyihasi mavsum
faolyatining oqibatlari
asosiy adabiyotlar
fakulteti ahborot
ahborot havfsizligi
havfsizligi kafedrasi
fanidan bo’yicha
fakulteti iqtisodiyot
boshqaruv fakulteti
chiqarishda boshqaruv
ishlab chiqarishda
iqtisodiyot fakultet
multiservis tarmoqlari
fanidan asosiy
Uzbek fanidan
mavzulari potok
asosidagi multiservis
'aliyyil a'ziym
billahil 'aliyyil
illaa billahil
quvvata illaa
falah' deganida
Kompyuter savodxonligi
bo’yicha mustaqil
'alal falah'
Hayya 'alal
'alas soloh
Hayya 'alas
mavsum boyicha


yuklab olish