Microbiology and Deterioration of Milk and Milk Products



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Bog'liq
CNDMS401.L3

Dye reduction test

  • Advantages
    • Simple, rapid, and inexpensive
    • Only viable cells actively reduce the dyes
  • Disadvantages
    • Not all organisms reduce the dyes equally
    • Not applicable to food specimens that contain reductive enzymes

Direct microscopic count

  • Method
  • Deliver 0.01 ml of milk on slide ,air dry
  • Stain with LW (Levowitz- Weber )stain for 10 min.
  • Count bacterial clumps with the microscope
  • Reading results:
  • Bacterial clump: dark blue
  • Protein, Leucocytes and somatic cells :light blue background
  • Fat globules : colorless
  • Dirt: brown
  • * clump : is one cell or group of cells of same type separated by a distance from other clumps or cells

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