Nistatin SMSN dan 0,0100 g olib dimetilformamidda 6030 Tb/ml miqdordagi
boshlang‘ich eritma hosil bo‘lgunicha eritiladi. Eritmani rn 6,0 bo‘lgan 5% li
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dimetilformamid saqlagan fosfat buferi eritmasi (FB 6,0+ DFA) bilan 10 Tb/ml(C1), 20
Tb/ml(C2), 40 Tb/ml miqdordagi konsentratsiyali eritmalar hosil bo‘lguncha suyultiriladi. Bu
jarayon quyidagi sxemaga asosan bajariladi:
0,0100 + 10,0 ml DFA 6030 Tb/ml
׀→ 1,0 ml + 5,03 (FB 6,0 + DFA) 1000 Tb/ml
׀→ 1,0 ml + 24,0 ml (FB 6,0 + DFA) 40 Tb/ml
׀→ 1,0 ml + 1,0 ml (FB 6,0 + DFA) 20 Tb/ml
׀→ 1,0 ml + 1,0 ml (FB 6,0 + DFA) 10 Tb/ml
Do'stlaringiz bilan baham: