Immobilization of Bacillus subtilis enzymes living on the surface of cotton

• Object of research: Bacillus subtilis strain living on the surface of cotton.
• Purpose and main tasks .
• The purpose of the study consists of immobilization of enzymes of the Bacillus subtilis strains, which lives on the surface of cotton.
• The main tasks of research are:
• 1. Effect of different factors on growth of Bacillus subtilis and α-amylase production
• 2. Effect of sodium alginate concentration during immobilization in alginate gel
• 3. Effect of concentration calcium chloride during immobilization in alginate gel
• 4.Effect of curing time of calcium alginate beads
• 5. Effect of pH and temperature on α-amylase activity
• 6. Effect of bead size on rate of starch hydrolysis
• 7. Reusability of immobilized α-amylase