Март-Апрель 2021
ДИСТАНЦИОННЫЕ ВОЗМОЖНОСТИ И ДОСТИЖЕНИЯ НАУКИ
Eagles Medium/ Ham’s F12 (DMEM/F12) (Gibco) supplemented with 10% fetal bovine serum
(FBS) (Gibco), 1% antibiotic/antimycotic (Gibco). HEK 293 cells were grown in minimum essential
medium (MEM) (Gibco) containing 5% FBS and 1% antibiotic/antimycotic. HUVECs were cultured
in Endothelial Cell Basal Medium-2 (EBM-2) (Lonza) supplemented with 2% FBS (Lonza) and
Endothelial Cell Growth Media-2 (EGM-2) SingleQuots Kit (Lonza). HUVECs were used at passage
five for all experiments. SUM149 cells were grown in DMEM/F12 supplemented with 5% FBS, 1%
antibiotic/antimycotic, 10 μg/mL insulin (Sigma-Aldrich) and 0.5 μg/mL hydrocortisone (Sigma-
Aldrich). All cells were maintained at 37
°
C and 5% CO2.
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