Antioxidants production of Aspergillus spp from Universitas Indonesia Culture Collection (uicc)

MATERIALS AND METHODS 
Microorganisms. 
Twelve strains of 
Aspergillus
spp. obtained from Universitas Indonesia Culture Collection 
(UICC) were selected for this study. 
Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017 (ISCPMS2017)
AIP Conf. Proc. 2023, 020169-1–020169-4; https://doi.org/10.1063/1.5064166
Published by AIP Publishing. 978-0-7354-1741-0/$30.00
020169-1

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Antioxidants Fermentation from 12 
Aspergillus
 spp. 
Antioxidants fermentation was conducted in Erlenmeyer 
flask of 250 mL containing 50 mL MYPG medium 0.3% malt extract; 0.3% yeast extract; 0.5% peptone, and 1% 
glucose at pH 6.8. One mL inoculum (3.5 - 4 x 10
7
cfu/mL) was inoculated into the fermentation medium and then 
incubated for 14 days at room temperature (26-29 °C) without shaking, the experiment was carried out in triplicates 
[
5
]
Extraction with Ethyl Acetate.
The culture filtrate was separated from mycelium using Whatmann no.1 filter 
paper and a vacuum pump. The culture filtrate was mixed with ethyle acetate the equal amount. The mixture was 
then further shaked and put into a separatory funnel until an ethyl acetate layer was formed. The ethyl acetate 
extract was filtered and dehydrated with natrium sulfate 1%, followed by evaporation in an evaporator machine at 
30
°C
 
[7].
 
Antioxidants Analysis Using Modified TLC Method [
5].
 
Silica gel pre-layer alumunium plate was first dried 
in 30 °C for 10 minutes to activate the silica gel. Each sample was solved in 1 mL ethyl acetate. After the TLC plate 
was activated, about five µL sample and standard was spotted and developed in a saturated developer solvent 
chloroform : methanol (88 : 12). The spotting process was carried out in a range 1.5 cm x 1.5 cm x 1.5 cm with 
interval of 1.5 cm. After the developer solvent stopped to move, it was removed from the container and dried in 
open air for 15 minutes. The TLC result was observed under UV light with 254 wave length. The Rf value from the 
chromatogram stains was determined by measuring the comparison of the distance length of a stain with its 
developer solvent from the spotted points.